University of Oulu

E. Perevedentseva, N. Ali, Y.-C. Lin, A. Karmenyan, C.-C. Chang, O. Bibikova, I. Skovorodkin, R. Prunskaite-Hyyryläinen, S. J. Vainio, M. Kinnunen, and C.-L. Cheng, "Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems," Biomed. Opt. Express 11, 5872-5885 (2020)

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

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Author: Perevedentseva, E.1,2; Ali, N.3; Lin, Y.-C.1;
Organizations: 1Department of Physics, National Dong Hwa University, Hualien, 97401, Taiwan
2P. N. Lebedev Physics Institute of Russian Academy of Sciences, Moscow, 119991, Russia
3Biocenter Oulu, Infotech Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, P.O.Box 8000 FI-90014 Oulu,Finland
4Faculty of Information Technology and Electrical Engineering, University of Oulu, P.O. Box 8000 FI-90014 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2020112593002
Language: English
Published: Optical Society of America, 2020
Publish Date: 2020-11-25
Description:

Abstract

In the present work, we report the imaging of Au nanostars nanoparticles (AuNSt) and their multifunctional applications in biomedical research and theranostics applications. Their optical and spectroscopic properties are considered for the multimodal imaging purpose. The AuNSt are prepared by the seed-meditated method and characterized for use as an agent for bio-imaging. To demonstrate imaging with AuNSt, penetration and localization in different biological models such as cancer cell culture (A549 lung carcinoma cell), 3D tissue model (multicellular tumor spheroid on the base of human oral squamous carcinoma cell, SAS) and murine skin tissue are studied. AuNSt were visualized using fluorescence lifetime imaging (FLIM) at two-photon excitation with a pulse duration 140 fs, repetition rate 80 MHz and 780 nm wavelength femtosecond laser. Strong emission of AuNSt at two-photon excitation in the near infrared range and fluorescence lifetime less than 0.5 ns were observed. It allows using AuNSt as a fluorescent marker at two-photon fluorescence microscopy and lifetime imaging (FLIM). It was shown that AuNSt can be observed inside a thick sample (tissue and its model). This is the first demonstration using AuNSt as an imaging agent for FLIM at two-photon excitation in biosystems. Increased scattering of near-infrared light upon excitation of AuNSt surface plasmon oscillation was also observed and rendered using a possible contrast agent for optical coherence tomography (OCT). AuNSt detection in a biological system using FLIM is compared with OCT on the model of AuNSt penetrating into animal skin. The AuNSt application for multimodal imaging is discussed.

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Series: Biomedical optics express
ISSN: 2156-7085
ISSN-E: 2156-7085
ISSN-L: 2156-7085
Volume: 11
Issue: 10
Pages: 5872 - 5885
DOI: 10.1364/BOE.401462
OADOI: https://oadoi.org/10.1364/BOE.401462
Type of Publication: A1 Journal article – refereed
Field of Science: 318 Medical biotechnology
1182 Biochemistry, cell and molecular biology
Subjects:
Funding: CLC thanks the Ministry of Science and Technology (MoST) of Taiwan for financially supporting this project (MOST 106-2112-M-259 -009 -MY3). This work was supported to SV by Academy of Finland Grants (315030, 304347), Sigrid Jusélius and Finnish Cancer Research Foundations and H2020-FETOPEN-2018-019-2020-01 projects MindGap GA 829040 and Gladiator (GA 828837). MK thanks the Academy of Finland (318103) for financially support this project.
Academy of Finland Grant Number: 315030
304347
318103
Detailed Information: 315030 (Academy of Finland Funding decision)
304347 (Academy of Finland Funding decision)
318103 (Academy of Finland Funding decision)
Copyright information: © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.