University of Oulu

Teemu Myllylä, Mika Kaakinen, Erkki Vihriälä, Jari Jukkola, Zuomin Zhao, Hany Ferdinando, Vesa Korhonen, Vesa Kiviniemi, Lauri Eklund, "Functional NIRS study of blood brain barrier disruption when induced by focused ultrasound and intra-arterial mannitol infusion," Proc. SPIE 11363, Tissue Optics and Photonics, 113630T (5 May 2020); doi: 10.1117/12.2555843

Functional NIRS study of blood brain barrier disruption when induced by focused ultrasound and intra-arterial mannitol infusion

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Author: Myllylä, Teemu1,2; Kaakinen, Mika3; Vihriälä, Erkki2;
Organizations: 1University of Oulu, Research Unit of Medical Imaging, Physics and Technology, Oulu, Finland
2University of Oulu, Optoelectronics and Measurement Techniques Unit, Oulu, Finland
3Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu, Finland
4University of Oulu, Reseah Unit of Medical Imaging, Physics and Technology, Oulu, Finland
5Oulu University Hospital, Department of Diagnostic Radiology, Medical Research Center (MRC), Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
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Language: English
Published: SPIE, 2020
Publish Date: 2020-11-25


Enhancing brain fluid movement across blood brain barrier (BBB) has been recognized as a potential treatment of neurodegenerative diseases. Moreover, BBB opening is of high interest also in brain drug delivery in the treatment of brain tumors/cancers. However, efficient therapies which are based on BBB opening are still limited because of insufficient understanding of mechanisms and safety issues. Currently, there are few promising methodologically diverse BBB opening approaches.

In this paper, we use functional near-infrared spectroscopy (fNIRS) for the first time for monitoring cerebral hemoglobin and water concentration changes during BBB opening in mouse brain by using two different techniques: intra-arterial mannitol infusion (IAM) and focused ultrasound (FUS). Both of these BBB opening techniques are already in clinical use but their hemo- and hydrodynamic implications have not been investigated from comparative aspect. Two fibre detectors were attached on both sides of the mouse brain and the source fibre was attached on middle of forehead. Further, by using a combination of three wavelengths 690nm, 830nm and 980nm, that have sufficient light penetration in the mouse brain, we can show average dynamics of hemoglobin and water in the whole brain, synchronized with BBB opening. To validate the level of BBB opening we used Evans blue dye and show its accumulation in the brain parenchyma tissue with the corresponding fNIRS responses.

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Series: Proceedings of SPIE
ISSN: 0277-786X
ISSN-E: 1996-756X
ISSN-L: 0277-786X
ISBN Print: 978-1-5106-3498-5
Article number: 113630T
DOI: 10.1117/12.2555843
Host publication: Tissue Optics and Photonics 2020, 6-10 April 2020, Online Only, France
Host publication editor: Tuchin, V. V.
Blondel, W. C. P. M.
Zalevsky, Z.
Conference: SPIE Photonics Europe
Type of Publication: B3 Article in conference proceedings
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
Copyright information: © 2020 SPIE. The Definitive Version of Record can be found online at: