Multiple crystal forms of human MacroD2 |
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Author: | Wazir, Sarah1; Maksimainen, Mirko M.1; Lehtiö, Lari1 |
Organizations: |
1Faculty of Biochemistry and Molecular Medicine, University of Oulu, P.O.Box 5400, Oulu, Finland, 90014, Finland |
Format: | article |
Version: | accepted version |
Access: | open |
Online Access: | PDF Full Text (PDF, 1.3 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe2020120399311 |
Language: | English |
Published: |
John Wiley & Sons,
2020
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Publish Date: | 2020-12-03 |
Description: |
AbstractMacroD2 is one of the three human macrodomain proteins characterized by their protein-linked mono-ADP-ribosyl-hydrolyzing activity. MacroD2 is a single-domain protein that contains a deep ADP-ribose-binding groove. In this study, new crystallization conditions for MacroD2 were found and three crystal structures of human MacroD2 in the apo state were solved in space groups P4₁2₁2, P43212 and P4₃, and refined at 1.75, 1.90 and 1.70 Å resolution, respectively. Structural comparison of the apo crystal structures with the previously reported crystal structure of MacroD2 in complex with ADP-ribose revealed conformational changes in the side chains of Val101, Ile189 and Phe224 induced by the binding of ADP-ribose in the active site. These conformational variations may potentially facilitate design efforts of a MacroD2 inhibitor. see all
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Series: |
Acta crystallographica. Section F, Structural biology communications |
ISSN: | 2053-230X |
ISSN-E: | 2053-230X |
ISSN-L: | 2053-230X |
Volume: | 76 |
Pages: | 477 - 482 |
DOI: | 10.1107/S2053230X20011309 |
OADOI: | https://oadoi.org/10.1107/S2053230X20011309 |
Type of Publication: |
A1 Journal article – refereed |
Field of Science: |
1182 Biochemistry, cell and molecular biology |
Subjects: | |
Funding: |
This work was funded by Academy of Finland (grant Nos.287063 and 294085) and by the Sigrid Juse ́lius Foundation. |
Academy of Finland Grant Number: |
287063 294085 |
Detailed Information: |
287063 (Academy of Finland Funding decision) 294085 (Academy of Finland Funding decision) |
Copyright information: |
© 2020 International Union of Crystallography. The final authenticated version is available online at https://doi.org/10.1107/s2053230x20011309. |