University of Oulu

Sinha, S.; Narjus-Sterba, M.; Tuomainen, K.; Kaur, S.; Seppänen-Kaijansinkko, R.; Salo, T.; Mannerström, B.; Al-Samadi, A. Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells. Int. J. Mol. Sci. 2020, 21, 6455.

Adipose-derived mesenchymal stem cells do not affect the invasion and migration potential of oral squamous carcinoma cells

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Author: Sinha, Snehadri1,2; Narjus-Sterba, Matilda1,3; Tuomainen, Katja1,3;
Organizations: 1Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland
2Helsinki University Central Hospital, 00290 Helsinki, Finland
3Translational Immunology Research Program (TRIMM), Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland
4Cancer and Translational Medicine Research Unit, University of Oulu, 90014 Oulu, Finland
5Medical Research Centre, Oulu University Hospital, 90014 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.4 MB)
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Language: English
Published: Multidisciplinary Digital Publishing Institute, 2020
Publish Date: 2020-12-14


Mesenchymal stem cells (MSCs) are commonly isolated from bone marrow and adipose tissue. Depending on the tissue of origin, MSCs have different characteristics and physiological effects. In various cancer studies, MSCs have been found to have either tumor-promoting or tumor-inhibiting action. This study investigated the effect of adipose tissue-MSCs (AT-MSCs) and bone marrow-MSCs (BM-MSCs) on global long interspersed nuclear element-1 (LINE-1) methylation, the expression level of microenvironment remodeling genes and cell proliferation, migration and invasion of oral tongue squamous cell carcinoma (OTSCC). Additionally, we studied the effect of human tongue squamous carcinoma (HSC-3)-conditioned media on LINE-1 methylation and the expression of microenvironment remodeling genes in AT-MSCs and BM-MSCs. Conditioned media from HSC-3 or MSCs did not affect LINE-1 methylation level in either cancer cells or MSCs, respectively. In HSC-3 cells, no effect of MSCs-conditioned media was detected on the expression of ICAM1, ITGA3 or MMP1. On the other hand, HSC-3-conditioned media upregulated ICAM1 and MMP1 expression in both types of MSCs. Co-cultures of AT-MSCs with HSC-3 did not induce proliferation, migration or invasion of the cancer cells. In conclusion, AT-MSCs, unlike BM-MSCs, seem not to participate in oral cancer progression.

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Series: International journal of molecular sciences
ISSN: 1661-6596
ISSN-E: 1422-0067
ISSN-L: 1661-6596
Volume: 21
Issue: 18
Article number: 6455
DOI: 10.3390/ijms21186455
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Funding: This research was funded by University of Helsinki project funding (WBS490302), the Doctoral Programme in Oral Sciences (FINDOS), the Doctoral Programme in Clinical Research (KLTO), Helsinki University Hospital State funding for university-level health research, Lasten Syöpäsäätiö Väre, the Finnish Dental Society Apollonia, Finnish-Norwegian medical foundation, the Selma and Maja-Lisa Selander Foundation, Sigrid Jusélius Foundation, the Cancer Society of Finland, the Oulu University Hospital MRC grant, and the Jane and Aatos Erkkos Foundation. Open access funding provided by University of Helsinki.
Copyright information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (