Mouse embryonic stem cell-derived ureteric bud progenitors induce nephrogenesis
|Author:||Tan, Zenglai1; Rak-Raszewska, Aleksandra1; Skovorodkin, Ilya1;|
1Center for Cell Matrix Research, Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Laboratory of Developmental Biology, Infotech Oulu, University of Oulu, Aapistie 5A, 90220 Oulu, Finland
2Borealis Biobank of Northern Finland, Oulu Central Hospital, 90220 Oulu, Finland
|Online Access:||PDF Full Text (PDF, 6.9 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe202102023514
Multidisciplinary Digital Publishing Institute,
|Publish Date:|| 2021-02-02
Generation of kidney organoids from pluripotent stem cells (PSCs) is regarded as a potentially powerful way to study kidney development, disease, and regeneration. Direct differentiation of PSCs towards renal lineages is well studied; however, most of the studies relate to generation of nephron progenitor population from PSCs. Until now, differentiation of PSCs into ureteric bud (UB) progenitor cells has had limited success. Here, we describe a simple, efficient, and reproducible protocol to direct differentiation of mouse embryonic stem cells (mESCs) into UB progenitor cells. The mESC-derived UB cells were able to induce nephrogenesis when co-cultured with primary metanephric mesenchyme (pMM). In generated kidney organoids, the embryonic pMM developed nephron structures, and the mESC-derived UB cells formed numerous collecting ducts connected with the nephron tubules. Altogether, our study established an uncomplicated and reproducible platform to generate ureteric bud progenitors from mouse embryonic stem cells.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1182 Biochemistry, cell and molecular biology
This work was supported financially by H2020 Marie Skłodowska-Curie Actions Innovative Training Network “RENALTRACT” (ID 642937), Academy of Finland (ID 315030, Centre of Excellence, ID 251314), Sigrid Jusélius Foundation, Cancer Research Foundation, and Finnish Cultural Foundation (personal grant to A.R.-R).
|Academy of Finland Grant Number:||
315030 (Academy of Finland Funding decision)
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).