University of Oulu

Saarinen, N.V.V.; Lehtonen, J.; Veijola, R.; Lempainen, J.; Knip, M.; Hyöty, H.; Laitinen, O.H.; Hytönen, V.P. Multiplexed High-Throughput Serological Assay for Human Enteroviruses. Microorganisms 2020, 8, 963.

Multiplexed high-throughput serological assay for human enteroviruses

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Author: Saarinen, Niila V. V.1; Lehtonen, Jussi1; Veijola, Riitta2;
Organizations: 1Faculty of Medicine and Health Technology, Tampere University, 33520 Tampere, Finland
2Department of Paediatrics, University of Oulu, 90570 Oulu, Finland
3Department of Paediatrics, University of Turku, 20520 Turku, Finland
4Pediatric Research Center, Children’s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland
5Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, 00029 Helsinki, Finland
6Fimlab Laboratories, 33520 Tampere, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 6.5 MB)
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Language: English
Published: Multidisciplinary Digital Publishing Institute, 2020
Publish Date: 2021-02-18


Immunological assays detecting antibodies against enteroviruses typically use a single enterovirus serotype as antigen. This limits the ability of such assays to detect antibodies against different enterovirus types and to detect possible type-specific variation in antibody responses. We set out to develop a multiplexed assay for simultaneous detection of antibodies against multiple enterovirus and rhinovirus types encompassing all human infecting species. Seven recombinant VP1 proteins from enteroviruses EV-A to EV-D and rhinoviruses RV-A to RV-C species were produced. Using Meso Scale Diagnostics U-PLEX platform we were able to study antibody reactions against these proteins as well as non-structural enterovirus proteins in a single well with 140 human serum samples. Adults had on average 33-fold stronger antibody responses to these antigens (p < 10⁻¹¹) compared to children, but children had less cross-reactivity between different enterovirus types. The results suggest that this new high-throughput assay offers clear benefits in the evaluation of humoral enterovirus immunity in children, giving more exact information than assays that are based on a single enterovirus type as antigen.

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Series: Microorganisms
ISSN: 2076-2607
ISSN-E: 2076-2607
ISSN-L: 2076-2607
Volume: 8
Issue: 6
Article number: 963
DOI: 10.3390/microorganisms8060963
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: This research was funded by Business Finland [Project THERDIAB 1843/31/2014 to N.V.V.S., H.H., V.P.H., O.H.L.], the Pirkanmaa Regional Fund, Tampere University doctoral school as well as Päivikki and Sakari Sohlberg Foundation [to N.V.V.S]. Vactech Ltd., ArcDia Ltd., JILAB Ltd. and Finnmedi Research Ltd. are acknowledged for their contribution within THERDIAB. We acknowledge Biocenter Finland for the infrastructure support.
Copyright information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (