University of Oulu

Rantala, S., Kaseva, J., Nukari, A. et al. Droplet vitrification technique for cryopreservation of a large diversity of blackcurrant (Ribes nigrum L.) cultivars. Plant Cell Tiss Organ Cult 144, 79–90 (2021).

Droplet vitrification technique for cryopreservation of a large diversity of blackcurrant (Ribes nigrum L.) cultivars

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Author: Rantala, Saija1,2; Kaseva, Janne3; Nukari, Anna4;
Organizations: 1Production Systems, Natural Resources Institute Finland (Luke), Survontie 9 A, 40500, Jyväskylä, Finland
2Faculty of Science, Ecology and Genetics Unit, University of Oulu, P.O.Box 3000, 90014, Oulu, Finland
3Natural Resources, Natural Resources Institute Finland (Luke), Tietotie 4, 31600, Jokioinen, Finland
4Production Systems, Natural Resources Institute Finland (Luke), Latokartanonkaari 9, 00790, Helsinki, Finland
5Natural Resources, Natural Resources Institute Finland (Luke), Survontie 9 A, 40500, Jyväskylä, Finland
6Production Systems, Natural Resources Institute Finland (Luke), Itäinen Pitkäkatu 4 A, 20520, Turku, Finland
7Boreal Plant Breeding Ltd., Myllytie 10, 31600, Jokioinen, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.7 MB)
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Language: English
Published: Springer Nature, 2020
Publish Date: 2021-02-22


The aim of plant gene banks is to preserve genetic resources selected based on their phenotypic, agronomic, historical or other cultural values for future utilization. In the present study the modified PVS2 droplet vitrification technique was tested and optimized for cryopreservation of a large diversity of blackcurrant (R. nigrum L.) accessions propagated in vitro and selected into a national gene bank core collection. Out of four accessions tested to optimize the method, three recovered and regenerated by 89–97% on average, but one recalcitrant in vitro line only by 25%. The tested post-cryopreservation recovery media with different macronutrient and growth regulator levels showed no generalized effect on regenerated shoots, but the effect of recovery media was different between cultivars. When the whole regeneration chain from cryopreservation via micropropagation to greenhouse conditions was tested, shoots at least 1 cm in length were found necessary for successful transfer ex vitro. The long-term cryopreservation of 22 blackcurrant accessions was finally conducted, with practices slightly modified from the tested protocol. The estimated recovery of shoot tips after 9 weeks in vitro was 17–94% with at least 75% recovery in seven accessions and at least 40% recovery in 19 out of 22 accessions. Only one accession had no cryopreservation success. The results demonstrated that the modified droplet vitrification technique is appropriate for a large diversity of blackcurrant accessions. However, cultivar-related differences and recovery procedures are to be considered for success in regeneration and ex vitro adaptation.

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Series: Plant cell, tissue and organ culture
ISSN: 0167-6857
ISSN-E: 1573-5044
ISSN-L: 0167-6857
Volume: 144
Issue: 1; SI
Pages: 79 - 90
DOI: 10.1007/s11240-020-01841-2
Type of Publication: A1 Journal article – refereed
Field of Science: 1183 Plant biology, microbiology, virology
Funding: Open access funding provided by Natural Resources Institute Finland (Luke). The authors are grateful to Mr Heikki Hokka, Ms Tiina Nieminen, Ms Riitta Toivakka and Ms Satu-Marja Virtanen for technical support in laboratory and greenhouse work. Our sincere appreciation is also expressed to Marjatta Uosukainen for advice during the various stages of the practical work and to all persons who helped to process plant material for cryopreservation. This research was partly funded by the European Commission, Directorate-General for Agriculture and Rural Development, under Council Regulation (EC) No 870/2004 through Action 071 AGRI GEN RES 870/2004 (RIBESCO), by The Ministry of Agriculture and Forestry of Finland, and by the foundations Maiju & Yrjö Rikalan Puutarhasäätiö (to SR) and Oiva Kuusisto Säätiö (to SR).
Copyright information: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit