University of Oulu

Maeba, Takahiro; Yonezawa, Tomoko; Ono, Mitsuaki; Tomono, Yasuko; Heljasvaara, Ritva; Pihlajaniemi, Taina; Inagawa, Kiichi; Oohashi, Toshitaka (2019) Collagen XVIII Deposition in the Basement Membrane Zone beneath the Newly Forming Epidermis during Wound Healing in Mice. Acta Medica Okayama 73(2), pp. 135-146,

Collagen XVIII deposition in the basement membrane zone beneath the newly forming epidermis during wound healing in mice

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Author: Maeba, Takahiro1; Yonezawa, Tomoko1; Ono, Mitsuaki1;
Organizations: 1Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700-8558, Japan
2Shigei Medical Research Institute, Okayama 701-0202, Japan
3Oulu Center for Cell-Matrix Research, Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu FIN-90014, Finland
4Center for Cancer Biomarkers CCBIO, Department of Biomedicine, University of Bergen, Bergen N-5009, Norway,
5Department of Plastic and Reconstructive Surgery, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 6.3 MB)
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Language: English
Published: Okayama University, 2019
Publish Date: 2021-03-04


The basement membrane (BM) is composed of various extracellular molecules and regulates tissue regeneration and maintenance. Here, we demonstrate that collagen XVIII was spatiotemporally expressed in the BM during skin wound healing in a mouse excisional wound-splinting model. Re-epithelialization was detected at days 3 and 6 post-wounding. The ultrastructure of epidermal BM was discontinuous at day 3, whereas on day 6 a continuous BM was observed in the region proximal to the wound edge. Immunohistochemistry demonstrated that collagen XVIII was deposited in the BM zone beneath newly forming epidermis in day 3 and 6 wounds. Laminin-332, known to be the earliest BM component appearing in wounds, was colocalized with collagen XVIII in the epidermal BM zone at days 3 and 6. The deposition of α1(IV) collagen and nidogen-1 in the epidermal BM zone occurred later than that of collagen XVIII. We also observed the short isoform of collagen XVIII in the epidermal BM zone at day 3 post-wounding. Collectively, our results suggested that collagen XVIII plays a role in the formation of the dermal-epidermal junction during re-epithelialization, and that it is the short isoform that is involved in the early phase of re-epithelialization.

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Series: Acta medica Okayama
ISSN: 0386-300X
ISSN-L: 0386-300X
Volume: 73
Issue: 2
Pages: 135 - 146
DOI: 10.18926/AMO/56649
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
3111 Biomedicine
Funding: This study was supported by a JSPS KAKENHI grant, no. JP17K11540.
Copyright information: Ⓒ 2019 by Okayama University Medical School. Published in this repository with the kind permission of the publisher.