University of Oulu

Shuo Liu, Jun Li, Teng Wang, Jiawen Xu, Zhipei Liu, Haobin Wang, Gong-Hong Wei, Alessandro Ianni, Thomas Braun & Shijing Yue (2019) Illumination of cell cycle progression by multi-fluorescent sensing system, Cell Cycle, 18:12, 1364-1378, DOI: 10.1080/15384101.2019.1618117

Illumination of cell cycle progression by multi-fluorescent sensing system

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Author: Liu, Shuo1,2; Li, Jun1,2; Wang, Teng1,2;
Organizations: 1State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China
2School of Medicine, Nankai University, Tianjin, China
3Department of Cardiac Development and Remodeling, Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany
4Union Gene Test & Health Management Center, Tianjin, China
5Department of Breast & Thyroid Surgery, The third people’s hospital of Chengdu; The Affiliated Hospital of Southwest Jiaotong University, Chengdu, China
6Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.6 MB)
Persistent link:
Language: English
Published: Taylor & Francis, 2019
Publish Date: 2021-03-04


Multi-fluorescent imaging of cell cycle progression is essential for the study of cell proliferation in vitro and in vivo. However, there remain challenges, particularly to image cell cycle progression in living cell with available imaging techniques due to lacking the suitable probe. Here, we design a triple fluorescent sensors system making the cell cycle progression visible. Multi-fluorescent sensor shows the proliferating or proliferated cells with different colors. We thus generate the construct and adenovirus to probe cell cycle progression in living cell lines and primary cardiomyocytes. Furthermore, we create the knock-in transgenic mouse to monitor cell cycle progression in vivo. Together, the system can be applied to investigate cell proliferation or cell cycle progression in living cells and animals.

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Series: Cell cycle
ISSN: 1538-4101
ISSN-E: 1551-4005
ISSN-L: 1538-4101
Volume: 18
Issue: 12
Pages: 1364 - 1378
DOI: 10.1080/15384101.2019.1618117
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: This work article was supported by the Natural Science Foundation of Tianjin, China (16JCYBJC43200), NSFC General Project (81773120), the Fundamental Research Funds for the Central Universities, Nankai University (63191214) to SY.
Copyright information: © 2019 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Cell Cycle on 26 May 2019, available online: