Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota |
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Author: | Saarinen, Markku Tapani1; Kärkkäinen, Olli2,3; Hanhineva, Kati2,4; |
Organizations: |
1DuPont Nutrition & Biosciences, Global Health & Nutrition Science, Kantvik, Finland 2Afekta Technologies Ltd., Kuopio, Finland 3School of Pharmacy, University of Eastern Finland, Kuopio, Finland
4Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland
5DuPont Nutrition & Biosciences, Genomics & Microbiome Science, St. Louis, MO, USA 6Institute of Biomedicine, Medical Research Center (MRC), University of Oulu, and University Hospital, Oulu, Finland 7Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, Poznan, Poland |
Format: | article |
Version: | published version |
Access: | open |
Online Access: | PDF Full Text (PDF, 1.9 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe202103117085 |
Language: | English |
Published: |
Springer Nature,
2020
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Publish Date: | 2021-03-11 |
Description: |
AbstractPolydextrose (PDX) is a branched glucose polymer, utilized as a soluble dietary fiber. Recently, PDX was found to have hypolipidemic effects and effects on the gut microbiota. To investigate these findings more closely, a non-targeted metabolomics approach, was exploited to determine metabolic alterations in blood and epididymal adipose tissue samples that were collected from C57BL/6 mice fed with a Western diet, with or without oral administration of PDX. Metabolomic analyses revealed significant differences between PDX- and control mice, which could be due to differences in diet or due to altered microbial metabolism in the gut. Some metabolites were found in both plasma and adipose tissue, such as the bile acid derivative deoxycholic acid and the microbiome-derived tryptophan metabolite indoxyl sulfate, both of which increased by PDX. Additionally, PDX increased the levels of glycine betaine and l-carnitine in plasma samples, which correlated negatively with plasma TG and positively correlated with bacterial genera enriched in PDX mice. The results demonstrated that PDX caused differential metabolite patterns in blood and adipose tissues and that one-carbon metabolism, associated with glycine betaine and l-carnitine, and bile acid and tryptophan metabolism are associated with the hypolipidemic effects observed in mice that were given PDX. see all
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Series: |
Scientific reports |
ISSN: | 2045-2322 |
ISSN-E: | 2045-2322 |
ISSN-L: | 2045-2322 |
Volume: | 10 |
Article number: | 21577 |
DOI: | 10.1038/s41598-020-78484-y |
OADOI: | https://oadoi.org/10.1038/s41598-020-78484-y |
Type of Publication: |
A1 Journal article – refereed |
Field of Science: |
3111 Biomedicine |
Subjects: | |
Copyright information: |
© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
https://creativecommons.org/licenses/by/4.0/ |