University of Oulu

Saarinen, M.T., Kärkkäinen, O., Hanhineva, K. et al. Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota. Sci Rep 10, 21577 (2020). https://doi.org/10.1038/s41598-020-78484-y

Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota

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Author: Saarinen, Markku Tapani1; Kärkkäinen, Olli2,3; Hanhineva, Kati2,4;
Organizations: 1DuPont Nutrition & Biosciences, Global Health & Nutrition Science, Kantvik, Finland
2Afekta Technologies Ltd., Kuopio, Finland
3School of Pharmacy, University of Eastern Finland, Kuopio, Finland
4Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland
5DuPont Nutrition & Biosciences, Genomics & Microbiome Science, St. Louis, MO, USA
6Institute of Biomedicine, Medical Research Center (MRC), University of Oulu, and University Hospital, Oulu, Finland
7Department of Gastroenterology and Metabolism, Poznan University of Medical Sciences, Poznan, Poland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.9 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe202103117085
Language: English
Published: Springer Nature, 2020
Publish Date: 2021-03-11
Description:

Abstract

Polydextrose (PDX) is a branched glucose polymer, utilized as a soluble dietary fiber. Recently, PDX was found to have hypolipidemic effects and effects on the gut microbiota. To investigate these findings more closely, a non-targeted metabolomics approach, was exploited to determine metabolic alterations in blood and epididymal adipose tissue samples that were collected from C57BL/6 mice fed with a Western diet, with or without oral administration of PDX. Metabolomic analyses revealed significant differences between PDX- and control mice, which could be due to differences in diet or due to altered microbial metabolism in the gut. Some metabolites were found in both plasma and adipose tissue, such as the bile acid derivative deoxycholic acid and the microbiome-derived tryptophan metabolite indoxyl sulfate, both of which increased by PDX. Additionally, PDX increased the levels of glycine betaine and l-carnitine in plasma samples, which correlated negatively with plasma TG and positively correlated with bacterial genera enriched in PDX mice. The results demonstrated that PDX caused differential metabolite patterns in blood and adipose tissues and that one-carbon metabolism, associated with glycine betaine and l-carnitine, and bile acid and tryptophan metabolism are associated with the hypolipidemic effects observed in mice that were given PDX.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 10
Article number: 21577
DOI: 10.1038/s41598-020-78484-y
OADOI: https://oadoi.org/10.1038/s41598-020-78484-y
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Subjects:
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