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Virus as renal functional genetic tools |
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| Author: | Sharma, Abhishek1; Cattavarayane, Sandhanakrishnan2 |
| Organizations: |
1Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Finland 2Department of Veterinary Biosciences, Section of Pathology, Faculty of Veterinary Medicine, University of Helsinki, Helsinki |
| Format: | article |
| Version: | accepted version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 0.2 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe202103258321 |
| Language: | English |
| Published: |
Humana Press,
2019
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| Publish Date: | 2021-03-25 |
| Description: |
AbstractViral vectors enable efficient transfection of ectopic DNA into hard to transfect cells. Viral vectors are normally used to obtain permanent modification of target cells, and tissues expect for the cases where integrase-deficient viruses are used. Here we describe a method to stably transfect metanephric mesenchyme cells isolated from the murine embryonic kidney at day E11.5. Using this method, it is possible to transfect hard to transfect cells and successfully evade host tissue immune response. Due to these advantages, this method has become one of the most frequently used in generating stable cell line, manipulation of tissues, and gene therapy. see all
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| Series: |
Methods in molecular biology |
| ISSN: | 1064-3745 |
| ISSN-E: | 1940-6029 |
| ISSN-L: | 1064-3745 |
| ISBN: | 978-1-4939-9021-4 |
| ISBN Print: | 978-1-4939-9020-7 |
| Volume: | 1926 |
| Pages: | 143 - 149 |
| DOI: | 10.1007/978-1-4939-9021-4_12 |
| OADOI: | https://oadoi.org/10.1007/978-1-4939-9021-4_12 |
| Host publication: |
Kidney organogenesis : methods in molecular biology |
| Host publication editor: |
Vainio, Seppo |
| Type of Publication: |
A3 Book chapter |
| Field of Science: |
1182 Biochemistry, cell and molecular biology 1184 Genetics, developmental biology, physiology |
| Subjects: | |
| Copyright information: |
© Springer Science+Business Media, LLC, part of Springer Nature 2019. This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at https://doi.org/10.1007/978-1-4939-9021-4_12.
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