University of Oulu

Sharma A., Cattavarayane S. (2019) Virus as Renal Functional Genetic Tools. In: Vainio S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY.

Virus as renal functional genetic tools

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Author: Sharma, Abhishek1; Cattavarayane, Sandhanakrishnan2
Organizations: 1Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Finland
2Department of Veterinary Biosciences, Section of Pathology, Faculty of Veterinary Medicine, University of Helsinki, Helsinki
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.2 MB)
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Language: English
Published: Humana Press, 2019
Publish Date: 2021-03-25


Viral vectors enable efficient transfection of ectopic DNA into hard to transfect cells. Viral vectors are normally used to obtain permanent modification of target cells, and tissues expect for the cases where integrase-deficient viruses are used. Here we describe a method to stably transfect metanephric mesenchyme cells isolated from the murine embryonic kidney at day E11.5. Using this method, it is possible to transfect hard to transfect cells and successfully evade host tissue immune response. Due to these advantages, this method has become one of the most frequently used in generating stable cell line, manipulation of tissues, and gene therapy.

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Series: Methods in molecular biology
ISSN: 1064-3745
ISSN-E: 1940-6029
ISSN-L: 1064-3745
ISBN: 978-1-4939-9021-4
ISBN Print: 978-1-4939-9020-7
Volume: 1926
Pages: 143 - 149
DOI: 10.1007/978-1-4939-9021-4_12
Host publication: Kidney organogenesis : methods in molecular biology
Host publication editor: Vainio, Seppo
Type of Publication: A3 Book chapter
Field of Science: 1182 Biochemistry, cell and molecular biology
1184 Genetics, developmental biology, physiology
Copyright information: © Springer Science+Business Media, LLC, part of Springer Nature 2019. This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at