Tuuli Ahlstrand, Heidi Tuominen, Arzu Beklen, Annamari Torittu, Jan Oscarsson, Raija Sormunen, Marja T. Pöllänen, Perttu Permi & Riikka Ihalin (2017) A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8, Virulence, 8:2, 115-134, DOI: 10.1080/21505594.2016.1216294
A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8
|Author:||Ahlstrand, Tuuli1; Tuominen, Heidi1; Beklen, Arzu1;|
1Department of Biochemistry, University of Turku, Turku, Finland
2Oral Microbiology, Department of Odontology, Umeå, University, Umeå, Sweden
3Biocenter Oulu and Department of Pathology, University of Oulu, Oulu Finland
4Institute of Dentistry, University of Turku, Turku, Finland
5Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Helsinki, Finland
6Department of Biological and Environmental Sciences, Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland
7Department of Chemistry, Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland
|Online Access:||PDF Full Text (PDF, 1.6 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe202103258342
|Publish Date:|| 2021-03-25
Intrinsically disordered proteins (IDPs) do not have a well-defined and stable 3-dimensional fold. Some IDPs can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. A novel outer membrane lipoprotein, bacterial interleukin receptor I (BilRI) of the opportunistic oral pathogen Aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (IL)-1β. Because the amino acid sequence of the novel lipoprotein resembles that of fibrinogen binder A of Haemophilus ducreyi, BilRI could have the potential to bind other proteins, such as host matrix proteins. However, from the tested host matrix proteins, BilRI interacted with neither collagen nor fibrinogen. Instead, the recombinant non-lipidated BilRI, which was intrinsically disordered, bound various pro/anti-inflammatory cytokines, such as IL-8, tumor necrosis factor (TNF)-α, interferon (IFN)-γ and IL-10. Moreover, BilRI played a role in the in vitro sensing of IL-1β and IL-8 because low concentrations of cytokines did not decrease the amount of extracellular DNA in the matrix of bilRI− mutant biofilm as they did in the matrix of wild-type biofilm when the biofilms were exposed to recombinant cytokines for 22 hours. BilRI played a role in the internalization of IL-1β in the gingival model system but did not affect either IL-8 or IL-6 uptake. However, bilRI deletion did not entirely prevent IL-1β internalization, and the binding of cytokines to BilRI was relatively weak. Thus, BilRI might sequester cytokines on the surface of A. actinomycetemcomitans to facilitate the internalization process in low local cytokine concentrations.
|Pages:||115 - 134|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1183 Plant biology, microbiology, virology
This work was supported by the Academy of Finland grants 265609 and 272960 to RI and 288235 to PP and the Central Foundation of Finnish Cultural Foundation to TA.
© 2017 Tuuli Ahlstrand, Heidi Tuominen, Arzu Beklen, Annamari Torittu, Jan Oscarsson, Raija Sormunen, Marja T. Pöllänen, Perttu Permi, and Riikka Ihalin. Published with license by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.