University of Oulu

Saarela U., Skovorodkin I. (2019) Time-Lapse Technologies and 4D Imaging of Kidney Development. In: Vainio S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY.

Time-lapse technologies and 4D imaging of kidney development

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Author: Saarela, Ulla1,2,3; Skovorodkin, Ilya1,2,3
Organizations: 1University of Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu, Finland
2Laboratory of Developmental Biology, Biocenter Oulu and InfoTech, Oulu, Finland
3Oulu Center for Cell Matrix Research, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.2 MB)
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Language: English
Published: Humana Press, 2019
Publish Date: 2021-03-25


Time-lapse imaging is a technique of frequent imaging and following a course of a process. Because the development of the embryonic kidney can proceed ex vivo after dissection, it is possible to study the morphogenesis by culturing the kidney in the onstage incubator of a microscope and follow the developmental process by imaging. Confocal microscopes and other three-dimensional imaging systems offer the possibility for tracking the development process in four dimensions—3D and the time.

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Series: Methods in molecular biology
ISSN: 1064-3745
ISSN-E: 1940-6029
ISSN-L: 1064-3745
ISBN: 978-1-4939-9021-4
ISBN Print: 978-1-4939-9020-7
Volume: 1926
Pages: 163 - 167
DOI: 10.1007/978-1-4939-9021-4_14
Host publication: Kidney organogenesis : methods and protocols
Host publication editor: Vainio, Seppo
Type of Publication: A3 Book chapter
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: This work was supported financially by the Suomen Akatemia (Academy of Finland) (206038, 121647, 250900, 260056; Centre of Excellence grant 2012-2017 251314), Munuaissäätiö—Finnish Kidney and Liver Association, the Sigrid Juséliuksen Säätiö, Novo Nordisk, Syöpäjärjestöt (Cancer Society of Finland), the European Community’s Seventh Framework Programme (FP7/2007-2013; grant FP7-HEALTH-F5-2012-INNOVATION-1 EURenOmics 305608), and H2020 Marie Skłodowska-Curie Actions Innovative Training Network “RENALTRACT” Project ID 642937.
EU Grant Number: (305608) EURENOMICS - European Consortium for High-Throughput Research in Rare Kidney Diseases
(642937) RENALTRACT - Development and disease of the renal tract
Academy of Finland Grant Number: 250900
Detailed Information: 250900 (Academy of Finland Funding decision)
121647 (Academy of Finland Funding decision)
260056 (Academy of Finland Funding decision)
251314 (Academy of Finland Funding decision)
Copyright information: © Springer Science+Business Media, LLC, part of Springer Nature 2019. This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at