Time-lapse technologies and 4D imaging of kidney development |
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Author: | Saarela, Ulla1,2,3; Skovorodkin, Ilya1,2,3 |
Organizations: |
1University of Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu, Finland 2Laboratory of Developmental Biology, Biocenter Oulu and InfoTech, Oulu, Finland 3Oulu Center for Cell Matrix Research, Oulu, Finland |
Format: | article |
Version: | accepted version |
Access: | open |
Online Access: | PDF Full Text (PDF, 0.2 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe202103258490 |
Language: | English |
Published: |
Humana Press,
2019
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Publish Date: | 2021-03-25 |
Description: |
AbstractTime-lapse imaging is a technique of frequent imaging and following a course of a process. Because the development of the embryonic kidney can proceed ex vivo after dissection, it is possible to study the morphogenesis by culturing the kidney in the onstage incubator of a microscope and follow the developmental process by imaging. Confocal microscopes and other three-dimensional imaging systems offer the possibility for tracking the development process in four dimensions—3D and the time. see all
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Series: |
Methods in molecular biology |
ISSN: | 1064-3745 |
ISSN-E: | 1940-6029 |
ISSN-L: | 1064-3745 |
ISBN: | 978-1-4939-9021-4 |
ISBN Print: | 978-1-4939-9020-7 |
Volume: | 1926 |
Pages: | 163 - 167 |
DOI: | 10.1007/978-1-4939-9021-4_14 |
OADOI: | https://oadoi.org/10.1007/978-1-4939-9021-4_14 |
Host publication: |
Kidney organogenesis : methods and protocols |
Host publication editor: |
Vainio, Seppo |
Type of Publication: |
A3 Book chapter |
Field of Science: |
1182 Biochemistry, cell and molecular biology |
Subjects: | |
Funding: |
This work was supported financially by the Suomen Akatemia (Academy of Finland) (206038, 121647, 250900, 260056; Centre of Excellence grant 2012-2017 251314), Munuaissäätiö—Finnish Kidney and Liver Association, the Sigrid Juséliuksen Säätiö, Novo Nordisk, Syöpäjärjestöt (Cancer Society of Finland), the European Community’s Seventh Framework Programme (FP7/2007-2013; grant FP7-HEALTH-F5-2012-INNOVATION-1 EURenOmics 305608), and H2020 Marie Skłodowska-Curie Actions Innovative Training Network “RENALTRACT” Project ID 642937. |
EU Grant Number: |
(305608) EURENOMICS - European Consortium for High-Throughput Research in Rare Kidney Diseases (642937) RENALTRACT - Development and disease of the renal tract |
Academy of Finland Grant Number: |
250900 121647 260056 251314 |
Detailed Information: |
250900 (Academy of Finland Funding decision) 121647 (Academy of Finland Funding decision) 260056 (Academy of Finland Funding decision) 251314 (Academy of Finland Funding decision) |
Copyright information: |
© Springer Science+Business Media, LLC, part of Springer Nature 2019. This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at https://doi.org/10.1007/978-1-4939-9021-4_14. |