University of Oulu

Tytgat, H.L.P., Rasinkangas, P., Ritari, J. et al. Selection and characterization of a SpaCBA pilus-secreting food-grade derivative of Lacticaseibacillus rhamnosus GG. Appl Microbiol Biotechnol 105, 1123–1131 (2021). https://doi.org/10.1007/s00253-020-11051-7

Selection and characterization of a SpaCBA pilus-secreting food-grade derivative of Lacticaseibacillus rhamnosus GG

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Author: Tytgat, Hanne L. P.1; Rasinkangas, Pia2,3; Ritari, Jarmo2,4;
Organizations: 1Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
2Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
3DuPont Nutrition & Biosciences, Kantvik, Finland
4Finnish Red Cross Blood Service, Helsinki, Finland
5University of Oulu, Oulu, Finland
6Functional Genomics Center Zurich, ETH Zurich, Zurich, Switzerland
7Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.4 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe202103308806
Language: English
Published: Springer Nature, 2021
Publish Date: 2021-03-30
Description:

Abstract

Many studies have established the functional properties of Lacticaseibacillus rhamnosus GG, previously known as Lactobacillus rhamnosus GG, marketed worldwide as a probiotic. The extraordinary capacity of L. rhamnosus GG to bind to human mucus and influence the immune system especially stand out. Earlier, we have shown the key role of its SpaCBA sortase-dependent pili encoded by the spaCBA-srtC1 gene cluster herein. These heterotrimeric pili consist of a shaft pilin SpaA, a basal pilin SpaB, and tip pilin SpaC that contains a mucus-binding domain. Here, we set out to characterize a food-grade non-GMO mutant of L. rhamnosus GG, strain PA11, which secretes its pilins, rather than coupling them to the cell surface, due to a defect in the housekeeping sortase A. The sortase-negative strain PA11 was extensively characterized using functional genomics and biochemical approaches and found to secrete the SpaCBA pili into the supernatant. Given the functional importance and uniqueness of the mucus-binding pili of L. rhamnosus GG, strain PA11 offers novel opportunities towards the characterization and further therapeutic application of SpaCBA pili and their low-cost, large-scale production.

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Series: Applied microbiology and biotechnology
ISSN: 0175-7598
ISSN-E: 1432-0614
ISSN-L: 0175-7598
Volume: 105
Issue: 3
Pages: 1123 - 1131
DOI: 10.1007/s00253-020-11051-7
OADOI: https://oadoi.org/10.1007/s00253-020-11051-7
Type of Publication: A1 Journal article – refereed
Field of Science: 318 Medical biotechnology
3111 Biomedicine
Subjects:
Funding: This project, including the efforts of PR, FPD, and JRitari were funded by the European Research Council (grant 250172, Microbes Inside to WMdV) and Academy of Finland (grants 252123 for FPD, 299749 for JReunanen, and 137389 for WMdV). The work of HPLT and WMdV was partly funded by the SIAM Gravity Grant 024.002.002 and Spinoza Award of the Netherlands Organization for Scientific Research of WMdV.
Academy of Finland Grant Number: 299749
Detailed Information: 299749 (Academy of Finland Funding decision)
Copyright information: © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
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