University of Oulu

Laakso, H, Ylä‐Herttuala, E, Sierra, A, et al. Docetaxel chemotherapy response in PC3 prostate cancer mouse model detected by rotating frame relaxations and water diffusion. NMR in Biomedicine. 2021; 34:e4483. https://doi.org/10.1002/nbm.4483

Docetaxel chemotherapy response in PC3 prostate cancer mouse model detected by rotating frame relaxations and water diffusion

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Author: Laakso, Hanne1; Ylä‐Herttuala, Elias1; Sierra, Alejandra1;
Organizations: 1A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland
2Department of Radiology, University of Turku, Turku, Finland
3Department of Radiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA
4Turku Center for Disease Modeling, University of Turku, Turku, Finland
5Turku PET Centre, University of Turku and Turku University Hospital, Turku, Finland
6Department of Future Technologies, University of Turku, Turku, Finland
7Medical Imaging Centre of Southwest Finland, Turku University Hospital, Turku, Finland
8Department of Oncology and Radiotherapy, Turku University Hospital, Turku, Finland
9Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland
10Department of Clinical Radiology, Oulu University Hospital, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 15.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021041610632
Language: English
Published: John Wiley & Sons, 2021
Publish Date: 2021-04-16
Description:

Abstract

MRI is a common method of prostate cancer diagnosis. Several MRI‐derived markers, including the apparent diffusion coefficient (ADC) based on diffusion‐weighted imaging, have been shown to provide values for prostate cancer detection and characterization. The hypothesis of the study was that docetaxel chemotherapy response could be picked up earlier with rotating frame relaxation times TRAFF2 and TRAFF4 than with the continuous wave T, adiabatic T, adiabatic T, T1, T2 or water ADC. Human PC3 prostate cancer cells expressing a red fluorescent protein were implanted in 21 male mice. Docetaxel chemotherapy was given once a week starting 1 week after cell implantation for 10 randomly selected mice, while the rest served as a control group (n = 11). The MRI consisted of relaxation along a fictitious field (RAFF) in the second (RAFF2) and fourth (RAFF4) rotating frames, T1 and T2, continuous wave T, adiabatic T and adiabatic T relaxation time measurements and water ADC. MRI was conducted at 7 T, once a week up to 4 weeks from cell implantation. The tumor volume was monitored using T2‐weighted MRI and optical imaging. The histology was evaluated after the last imaging time point. Significantly reduced RAFFn, T, T and conventional relaxation times 4 weeks after tumor implantation were observed in the treated tumors compared with the controls. The clearest short‐ and long‐term responses were obtained with T1, while no clear improvement in response to treatment was detected with novel methods compared with conventional methods or with RAFFn compared with all others. The tumor volume decreased after a two‐week time point for the treated group and increased significantly in the control group, which was supported by increasing red fluorescent light emission in the control tumors. Decreased relaxation times were associated with successful chemotherapy outcomes. The results indicate altered relaxation mechanisms compared with higher dose chemotherapies previously published.

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Series: NMR in biomedicine
ISSN: 0952-3480
ISSN-E: 1099-1492
ISSN-L: 0952-3480
Volume: 34
Issue: 4
Article number: e4483
DOI: 10.1002/nbm.4483
OADOI: https://oadoi.org/10.1002/nbm.4483
Type of Publication: A1 Journal article – refereed
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
3122 Cancers
Subjects:
Funding: Alejandra Sierra was funded by the Academy of Finland.
Copyright information: © 2021 The Authors. NMR in Biomedicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
  https://creativecommons.org/licenses/by-nc-nd/4.0/