Kyrklund, M., Kaski, H., Akhi, R., Nissinen, A. E., Kummu, O., Bergmann, U., Pussinen, P., Hörkkö, S., & Wang, C. (2021). Existence of natural mouse IgG mAbs recognising epitopes shared by malondialdehyde acetaldehyde adducts and Porphyromonas gingivalis. Innate Immunity, 27(2), 158–169. https://doi.org/10.1177/1753425920981133
Existence of natural mouse IgG mAbs recognising epitopes shared by malondialdehyde acetaldehyde adducts and Porphyromonas gingivalis
|Author:||Kyrklund, Mikael1,2; Kaski, Heidi1; Akhi, Ramin1,2;|
1Medical Microbiology and Immunology, Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Finland
2Medical Research Centre and Nordlab Oulu, University Hospital and University of Oulu, Finland
3Protein Analysis Core Facility, Biocentre Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Finland
4Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Finland
5Minerva Foundation Institute for Medical Research, Biomedicum Helsinki 2U, Finland
|Online Access:||PDF Full Text (PDF, 0.7 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2021041610643
|Publish Date:|| 2021-04-16
Natural Abs are produced by B lymphocytes in the absence of external Ag stimulation. They recognise self, altered self and foreign Ags, comprising an important first-line defence against invading pathogens and serving as innate recognition receptors for tissue homeostasis. Natural IgG Abs have been found in newborns and uninfected individuals. Yet, their physiological role remains unclear. Previously, no natural IgG Abs to oxidation-specific epitopes have been reported. Here, we show the cloning and characterisation of mouse IgG mAbs against malondialdehyde acetaldehyde (MAA)-modified low-density lipoprotein. Sequence analysis reveals high homology with germline genes, suggesting that they are natural. Further investigation shows that the MAA-specific natural IgG Abs cross-react with the major periodontal pathogen Porphyromonas gingivalis and recognise its principle virulence factors gingipain Kgp and long fimbriae. The study provides evidence that natural IgGs may play an important role in innate immune defence and in regulation of tissue homeostasis by recognising and removing invading pathogens and/or modified self-Ags, thus being involved in the development of periodontitis and atherosclerosis.
|Pages:||158 - 169|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This study was supported by the Research Fund of the Medical Research Center, University of Oulu and Oulu University Hospital, Research Fund of Oulu University Hospital/special state support for research, The Finnish Foundation for Cardiovascular Research and Aarne Koskelo Foundation. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
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