Smoking effect on the ultrastructural properties of cultured lung myofibroblasts
Lehtonen, Siri; Nurmos, Ninni-Ingrid; Karvonen, Henna M.; Lappi-Blanco, Elisa; Harju, Terttu; Sköld, Magnus; Kaarteenaho, Riitta (2020-12-30)
Siri Lehtonen, Ninni-Ingrid Nurmos, Henna M Karvonen, Elisa Lappi-Blanco, Terttu Harju, Magnus Sköld & Riitta Kaarteenaho (2021) Smoking effect on the ultrastructural properties of cultured lung myofibroblasts, Ultrastructural Pathology, 45:1, 37-48, DOI: 10.1080/01913123.2020.1858214
© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
https://creativecommons.org/licenses/by/4.0/
https://urn.fi/URN:NBN:fi-fe2021041910907
Tiivistelmä
Abstract
This study aimed at an ultrastructural characterization of myofibroblasts cultured from different compartments of lung from never-smokers and smokers with or without COPD. In addition, we evaluated the expression of alpha smooth muscle actin (α-SMA), a marker for myofibroblasts, and contractile properties. Stromal cells cultured from central and corresponding peripheral or only from peripheral lung of never-smokers, smokers without COPD and COPD patients were analyzed by transmission electron microscopy (TEM), immunoelectron microscopy (IEM), Western analysis and/or by collagen gel contraction assay. TEM revealed that myofibroblasts cultured from smokers and COPD had less prominent intracellular actin filaments. We also examined fibronexus (FNX), which is a typical ultrastructural feature of myofibroblasts, and observed that patients with COPD more frequently had tandem-like FNX as compared to other samples. Western analysis showed that the samples derived from the central lung of never-smokers expressed higher levels of α-SMA than those of smokers and COPD patients. Cells from central lung were less contractile than those from peripheral lung. We conclude that myofibroblasts have variable ultrastructural and functional properties based on their localization in the lung and, moreover, these properties are affected by both smoking history and COPD.
Kokoelmat
- Avoin saatavuus [31657]