University of Oulu

Arima, K., Lau, M., Zhao, M., Haruki, K., Kosumi, K., Mima, K., Gu, M., Väyrynen, J., Twombly, T., Baba, Y., Fujiyoshi, K., Kishikawa, J., Guo, C., Baba, H., Richards, W., Chan, A., Nishihara, R., Meyerhardt, J., Nowak, J., Giannakis, M., Fuchs, C., Ogino, S. (2020) Metabolic Profiling of Formalin-Fixed Paraffin-Embedded Tissues Discriminates Normal Colon from Colorectal Cancer. Molecular Cancer Research, 18 (6), 883-890. https://doi.org/10.1158/1541-7786.MCR-19-1091

Metabolic profiling of formalin-fixed paraffin-embedded tissues discriminates normal colon from colorectal cancer

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Author: Arima, Kota1,2,3; Lau, Mai Chan1; Zhao, Melissa1;
Organizations: 1Program in MPE Molecular Pathological Epidemiology, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA
2Department of Oncologic Pathology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA
3Department of Gastroenterological Surgery, Graduate School of Medical Science, Kumamoto University, Kumamoto, Japan
4Department of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA
5Cancer and Translational Medicine Research Unit, Medical Research Center Oulu, University of Oulu, and Oulu University Hospital, Oulu, Finland
6Division of Thoracic Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA
7Clinical and Translational Epidemiology Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA
8Division of Gastroenterology, Massachusetts General Hospital, Boston, MA
9Channing Division of Network Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA
10Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA
11Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA
12Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA
13Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, MA
14Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA
15Broad Institute of MIT and Harvard, Cambridge, MA
16Yale Cancer Center, New Haven, CT
17Department of Medicine, Yale School of Medicine, New Haven, CT
18Smilow Cancer Hospital, New Haven, CT
19Cancer Immunology and Cancer Epidemiology Programs, Dana-Farber Harvard Cancer Center, Boston, MA
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021042611944
Language: English
Published: American Association for Cancer Research, 2020
Publish Date: 2021-04-26
Description:

Abstract

Accumulating evidence suggests that metabolic reprogramming has a critical role in carcinogenesis and tumor progression. The usefulness of formalin-fixed paraffin-embedded (FFPE) tissue material for metabolomics analysis as compared with fresh frozen tissue material remains unclear. LC/MS-MS–based metabolomics analysis was performed on 11 pairs of matched tumor and normal tissues in both FFPE and fresh frozen tissue materials from patients with colorectal carcinoma. Permutation t test was applied to identify metabolites with differential abundance between tumor and normal tissues. A total of 200 metabolites were detected in the FFPE samples and 536 in the fresh frozen samples. The preservation of metabolites in FFPE samples was diverse according to classes and chemical characteristics, ranging from 78% (energy) to 0% (peptides). Compared with the normal tissues, 34 (17%) and 174 (32%) metabolites were either accumulated or depleted in the tumor tissues derived from FFPE and fresh frozen samples, respectively. Among them, 15 metabolites were common in both FFPE and fresh frozen samples. Notably, branched chain amino acids were highly accumulated in tumor tissues. Using KEGG pathway analyses, glyoxylate and dicarboxylate metabolism, arginine and proline, glycerophospholipid, and glycine, serine, and threonine metabolism pathways distinguishing tumor from normal tissues were found in both FFPE and fresh frozen samples. This study demonstrates that informative data of metabolic profiles can be retrieved from FFPE tissue materials.

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Series: Molecular cancer research
ISSN: 1541-7786
ISSN-E: 1557-3125
ISSN-L: 1541-7786
Volume: 18
Issue: 65
Pages: 883 - 890
DOI: 10.1158/1541-7786.MCR-19-1091
OADOI: https://oadoi.org/10.1158/1541-7786.MCR-19-1091
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Subjects:
Funding: This work was supported by NIH grants (P50 CA127003, to C.S. Fuchs; R01 CA118553, to C.S. Fuchs; R01 CA169141, to C.S. Fuchs; R01 CA137178, to A.T. Chan; K24 DK098311, to A.T. Chan; R35 CA197735, to S. Ogino; R01 CA151993, to S. Ogino; R21 CA230873, to S. Ogino, K07 CA190673, to R. Nishihara); by Nodal Award (2016-02) from the Dana-Farber Harvard Cancer Center (to S. Ogino); by Stand Up to Cancer Colorectal Cancer Dream Team Translational Research Grant (grant number SU2C-AACR-DT22-17; to C.S. Fuchs and M. Giannakis); and by grants from the Project P Fund, The Friends of the Dana-Farber Cancer Institute, Bennett Family Fund, and the Entertainment Industry Foundation through National Colorectal Cancer Research Alliance. Stand Up To Cancer is a division of the Entertainment Industry Foundation. Research grants are administered by the American Association for Cancer Research, the scientific partner of SU2C. K. Arima was supported by grants from Overseas Research Fellowship from Japan Society for the Promotion of Science (201860083). K. Haruki was supported by a fellowship grant from the Uehara Memorial Foundation and the Mitsukoshi Health and Welfare Foundation. K. Fujiyoshi was supported by a fellowship grant from the Uehara Memorial Foundation. A.T. Chan is a Stuart and Suzanne Steele MGH Research Scholar.
Copyright information: © 2020 American Association for Cancer Research.