University of Oulu

Bijay Ratna Shakya, Hanna-Riikka Teppo, Lassi Rieppo, Discrimination of melanoma cell lines with Fourier Transform Infrared (FTIR) spectroscopy, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Volume 254, 2021, 119665, ISSN 1386-1425,

Discrimination of melanoma cell lines with Fourier Transform Infrared (FTIR) spectroscopy

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Author: Shakya, Bijay Ratna1; Teppo, Hanna-Riikka2,3,4; Rieppo, Lassi1
Organizations: 1Research Unit of Medical Imaging, Physics and Technology, University of Oulu/Faculty of Medicine, Aapistie 5 A, 90220 Oulu, Finland
2Cancer Research and Translational Medicine Research Unit, University of Oulu, Aapistie 5 A, 90220 Oulu, Finland
3Department of Pathology, Oulu University Hospital, Oulu, Finland
4Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.3 MB)
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Language: English
Published: Elsevier, 2021
Publish Date: 2021-05-07


Among skin cancers, melanoma is the lethal form and the leading cause of death in humans. Melanoma begins in melanocytes and is curable at early stages. Thus, early detection and evaluation of its metastatic potential are crucial for effective clinical intervention. Fourier transform infrared (FTIR) spectroscopy has gained considerable attention due to its versatility in detecting biochemical and biological features present in the samples. Changes in these features are used to differentiate between samples at different stages of the disease. Previously, FTIR spectroscopy has been mostly used to distinguish between healthy and diseased conditions. With this study, we aim to discriminate between different melanoma cell lines based on their FTIR spectra. Formalin-fixed paraffin embedded samples from three melanoma cell lines (IPC-298, SK-MEL-30 and COLO-800) were used. Statistically significant differences were observed in the prominent spectral bands of three cell lines along with shifts in peak positions. A partial least square discriminant analysis (PLS-DA) model built for the classification of three cell lines showed an overall accuracy of 92.6% with a sensitivity of 85%, 95.75%, 96.54%, and specificity of 97.80%, 92.14%, 98.64% for the differentiation of IPC-298, SK-MEL-30, and COLO-800, respectively. The results suggest that FTIR spectroscopy can differentiate between different melanoma cell lines and thus potentially characterize the metastatic potential of melanoma.

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Series: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1386-1425
ISSN-E: 1873-3557
ISSN-L: 1386-1425
Volume: 254
Article number: 119665
DOI: 10.1016/j.saa.2021.119665
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
3126 Surgery, anesthesiology, intensive care, radiology
Copyright information: © 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (