University of Oulu

Ivanov, V., Marusik, Y., Pétillon, J. et al. Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae). Sci Rep 11, 2177 (2021).

Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)

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Author: Ivanov, Vladislav1; Marusik, Yuri2,3; Pétillon, Julien4;
Organizations: 1Department of Ecology and Genetics, University of Oulu, Oulu, Finland
2Institute for Biological Problems of the North, RAS, Magadan, Russia
3Department of Zoology and Entomology, University of the Free State, Bloemfontein, 9300, South Africa
4UMR CNRS ECOBIO, Université de Rennes 1, Rennes, France
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.1 MB)
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Language: English
Published: Springer Nature, 2021
Publish Date: 2021-05-19


Although species delimitation is often controversial, emerging DNA-based and classical morphology-based methods are rarely compared using large-scale samplings, even less in the case of widely distributed species that have distant, allopatric populations. In the current study, we examined species boundaries within two wolf spider species of the genus Pardosa (Araneae, Lycosidae), P. riparia and P. palustris. Wolf spiders constitute an excellent model for testing the relevance of traditional vs. modern methods in species and population delimitation because several closely related species are distributed over cross-continental geographic ranges. Allopatric populations of the two Pardosa species were sampled across Europe to Far East Russia (latitudinal range > 150°) and several dozen individuals were studied using morphological characters (morphometry of three measures for both sexes, plus five in males only and two in females only), DNA barcoding (COI sequencing) and double-digest restriction site associated DNA sequencing (ddRADseq). The results obtained allow for changing the taxonomic status of two Far East Russian populations to subspecies and ddRADseq proved to be a powerful tool for taxonomic research despite scarce sampling and inherent subjectivity of species delimitation in allopatry. Overall, this study pleads for both multi-criteria and more population-based studies in taxonomy.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 11
Issue: 1
Article number: 2177
DOI: 10.1038/s41598-021-81788-2
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
Funding: Funding was provided by “Suomen Akatemia”, Grant No. 277984 accorded to MM, “Kvantum Institute” (accorded to MM) and “Suomen Hyönteistieteellinen Seura” (personal grant to VI).
Academy of Finland Grant Number: 277984
Detailed Information: 277984 (Academy of Finland Funding decision)
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