Kreus M, Lehtonen S, Skarp S, Kaarteenaho R (2021) Extracellular matrix proteins produced by stromal cells in idiopathic pulmonary fibrosis and lung adenocarcinoma. PLoS ONE 16(4): e0250109. https://doi.org/10.1371/journal.pone.0250109
Extracellular matrix proteins produced by stromal cells in idiopathic pulmonary fibrosis and lung adenocarcinoma
|Author:||Kreus, Mervi1,2; Lehtonen, Siri2,3; Skarp, Sini4;|
1Research Unit of Internal Medicine, University of Oulu, Oulu, Finland
2Medical Research Center Oulu, Oulu University Hospital, Oulu, Finland
3Department of Obstetrics and Gynecology, Oulu University Hospital, Oulu, Finland
4Northern Finland Birth Cohorts, Infrastructure for Population Studies, Faculty of Medicine, University of Oulu, Oulu, Finland
|Online Access:||PDF Full Text (PDF, 1.7 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2021052631672
Public Library of Science,
|Publish Date:|| 2021-05-26
Idiopathic pulmonary fibrosis (IPF) and lung cancer share common risk factors, epigenetic and genetic alterations, the activation of similar signaling pathways and poor survival. The aim of this study was to examine the gene expression profiles of stromal cells from patients with IPF and lung adenocarcinoma (ADC) as well as from normal lung. The gene expression levels of cultured stromal cells derived from non-smoking patients with ADC from the tumor (n = 4) and the corresponding normal lung (n = 4) as well as from patients with IPF (n = 4) were investigated with Affymetrix microarrays. The expression of collagen type IV alpha 1 chain, periostin as well as matrix metalloproteinase-1 and -3 in stromal cells and lung tissues were examined with quantitative real-time reverse transcriptase polymerase chain reaction and immunohistochemistry, respectively. Twenty genes were similarly up- or down-regulated in IPF and ADC compared to control, while most of the altered genes in IPF and ADC were differently expressed, including several extracellular matrix genes. Collagen type IV alpha 1 chain as well as matrix metalloproteinases-1 and -3 were differentially expressed in IPF compared to ADC. Periostin was up-regulated in both IPF and ADC in comparison to control. All studied factors were localized by immunohistochemistry in stromal cells within fibroblast foci in IPF and stroma of ADC. Despite the similarities found in gene expressions of IPF and ADC, several differences were also detected, suggesting that the molecular changes occurring in these two lung illnesses are somewhat different.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
3121 General medicine, internal medicine and other clinical medicine
R.K. has received grants for the study group from Foundation of the Finnish Anti-Tuberculosis Association, the Research Foundation of the Pulmonary Diseases, Jalmari and Rauha Ahokas Foundation, the Research Foundation of North Finland, and a state subsidy of the Oulu University Hospital. M.K. has received grants for the scientific work from Väinö and Laina Kivi Foundation, the Foundation of the Finnish Anti-Tuberculosis Association, the Tampere Tuberculosis foundation, and the Research Foundation of the Pulmonary Diseases. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
© 2021 Kreus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.