University of Oulu

V.-P. Karjalainen, I. Kestilä, M.A. Finnilä, E. Folkesson, A. Turkiewicz, P. Önnerfjord, V. Hughes, J. Tjörnstrand, M. Englund, S. Saarakkala, Quantitative three-dimensional collagen orientation analysis of human meniscus posterior horn in health and osteoarthritis using micro-computed tomography, Osteoarthritis and Cartilage, Volume 29, Issue 5, 2021, Pages 762-772, ISSN 1063-4584, https://doi.org/10.1016/j.joca.2021.01.009

Quantitative three-dimensional collagen orientation analysis of human meniscus posterior horn in health and osteoarthritis using micro-computed tomography

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Author: Karjalainen, V.-P.1; Kestilä, I.1; Finnilä, M. A.1;
Organizations: 1Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
2Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden
3Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, Rheumatology and Molecular Skeletal Biology, Lund, Sweden
4Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, Orthopaedics Skane University Hospital, Lund, Sweden
5Department of Diagnostic Radiology, Oulu University Hospital, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.9 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021060935915
Language: English
Published: Elsevier, 2021
Publish Date: 2021-06-09
Description:

Summary

Objective: Knee osteoarthritis (OA) is associated with meniscal degeneration that may involve disorganization of the meniscal collagen fiber network. Our aims were to quantitatively analyze the microstructural organization of human meniscus samples in 3D using micro-computed tomography (μCT), and to compare the local microstructural organization between OA and donor samples.

Method: We collected posterior horns of both medial and lateral human menisci from 10 end-stage medial compartment knee OA patients undergoing total knee replacement (medial & lateral OA) and 10 deceased donors without knee OA (medial & lateral donor). Posterior horns were dissected and fixed in formalin, dehydrated in ascending ethanol concentrations, treated with hexamethyldisilazane (HMDS), and imaged with μCT. We performed local orientation analysis of collagenous microstructure in 3D by calculating structure tensors from greyscale gradients within selected integration window to determine the polar angle for each voxel.

Results: In donor samples, meniscus bundles were aligned circumferentially around the inner border of meniscus. In medial OA menisci, the organized structure of collagen network was lost, and main orientation was shifted away from the circumferential alignment. Quantitatively, medial OA menisci had the lowest mean orientation angle compared to all groups, −24° (95%CI -31 to −18) vs medial donor and −25° (95%CI -34 to −15) vs lateral OA.

Conclusions: HMDS-based μCT imaging enabled quantitative analysis of meniscal collagen fiber bundles and their orientations in 3D. In human medial OA menisci, the collagen disorganization was profound with overall lower orientation angles, suggesting collagenous microstructure disorganization as an important part of meniscus degradation.

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Series: Osteoarthritis and cartilage
ISSN: 1063-4584
ISSN-E: 1522-9653
ISSN-L: 1063-4584
Volume: 29
Issue: 5
Pages: 762 - 772
DOI: 10.1016/j.joca.2021.01.009
OADOI: https://oadoi.org/10.1016/j.joca.2021.01.009
Type of Publication: A1 Journal article – refereed
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
Subjects:
Funding: This project has received funding from the European Research Council funded this project under European Union's Horizon 2020 research and innovation programme and under European Union's Seventh Framework Programme (FP/2007–2013) (grant agreement No 336267). This work was also supported by the Swedish Research Council (Dnr 2014–2348), the Foundation for Research in Rheumatology (FOREUM) (018EnglundPreCl), the Greta and Johan Kock Foundation, the Swedish Rheumatism Association, The Österlund Foundation, The Governmental Funding of Clinical Research program within the National Health Service (ALF) in Sweden, and the Faculty of Medicine, Lund University, Sweden.
Copyright information: © 2021 The Author(s). Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
  https://creativecommons.org/licenses/by/4.0/