Erkoc-Biradli, F.Z., Ozgun, A., Öztürk-Öncel, M.Ö., Marcali, M., Elbuken, C., Bulut, O., Rasier, R. and Garipcan, B. (2021), Bioinspired hydrogel surfaces to augment corneal endothelial cell monolayer formation. J Tissue Eng Regen Med, 15: 244-255. https://doi.org/10.1002/term.3173
Bioinspired hydrogel surfaces to augment corneal endothelial cell monolayer formation
|Author:||Erkoc‐Biradli, Fatma Zehra1; Ozgun, Alp1; Öztürk‐Öncel, Meftune Özgen1;|
1(Bio)3 Research laboratory, Institute of Biomedical Engineering, Bogazici University, Istanbul, Turkey
2Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey
3Faculty of Biochemistry and Molecular Medicine, Faculty of Medicine, University of Oulu, Oulu, Finland
4Faculty of Civil Engineering, Istanbul Technical University, Istanbul, Turkey
5Department of Ophthalmology, Demiroglu Bilim University, Istanbul, Turkey
|Online Access:||PDF Full Text (PDF, 24.4 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2021070541114
John Wiley & Sons,
|Publish Date:|| 2022-01-15
Corneal endothelial cells (CECs) have limited proliferation ability leading to corneal endothelium (CE) dysfunction and eventually vision loss when cell number decreases below a critical level. Although transplantation is the main treatment method, donor shortage problem is a major bottleneck. The transplantation of in vitro developed endothelial cells with desirable density is a promising idea. Designing cell substrates that mimic the native CE microenvironment is a substantial step to achieve this goal. In the presented study, we prepared polyacrylamide (PA) cell substrates that have a microfabricated topography inspired by the dimensions of CECs. Hydrogel surfaces were prepared via two different designs with small and large patterns. Small patterned hydrogels have physiologically relevant hexagon densities (∼2000 hexagons/mm²), whereas large patterned hydrogels have sparsely populated hexagons (∼400 hexagons/mm²). These substrates have similar elastic modulus of native Descemet’s membrane (DM; ∼50 kPa) and were modified with Collagen IV (Col IV) to have biochemical content similar to native DM. The behavior of bovine corneal endothelial cells on these substrates was investigated and results show that cell proliferation on small patterned substrates was significantly (p = 0.0004) higher than the large patterned substrates. Small patterned substrates enabled a more densely populated cell monolayer compared to other groups (p = 0.001 vs. flat and p < 0.0001 vs. large patterned substrates). These results suggest that generating bioinspired surface topographies augments the formation of CE monolayers with the desired cell density, addressing the in vitro development of CE layers.
Journal of tissue engineering and regenerative medicine
|Pages:||244 - 255|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1182 Biochemistry, cell and molecular biology
This study was supported by the Bogazici University Research Fund by Grant nos. 11501 and 6701. Fatma Zehra Erkoc-Biradli would like to thank TUBITAK 2210-C Program of Domestic Graduate Scholarship in Priority Areas (2015/2) for their support. Caglar Elbuken acknowledges the support from The Science Academy, Turkey through the Young Scientist Award Program.
© 2021 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Erkoc-Biradli, F.Z., Ozgun, A., Öztürk-Öncel, M.Ö., Marcali, M., Elbuken, C., Bulut, O., Rasier, R. and Garipcan, B. (2021), Bioinspired hydrogel surfaces to augment corneal endothelial cell monolayer formation. J Tissue Eng Regen Med, 15: 244-255, which has been published in final form at https://doi.org/10.1002/term.3173. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.