University of Oulu

Muilu-Mäkelä, R.; Vuosku, J.; Häggman, H.; Sarjala, T. Polyamine Metabolism in Scots Pine Embryogenic Cells under Potassium Deficiency. Cells 2021, 10, 1244.

Polyamine metabolism in Scots pine embryogenic cells under potassium deficiency

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Author: Muilu-Mäkelä, Riina1; Vuosku, Jaana2; Häggman, Hely2;
Organizations: 1Natural Resources Institute Finland (Luke), 00791 Helsinki, Finland
2Ecology and Genetics Research Unit, University of Oulu, 90570 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 13 MB)
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Language: English
Published: Multidisciplinary Digital Publishing Institute, 2021
Publish Date: 2021-07-06


Polyamines (PA) have a protective role in maintaining growth and development in Scots pine during abiotic stresses. In the present study, a controlled liquid Scots pine embryogenic cell culture was used for studying the responses of PA metabolism related to potassium deficiency. The transcription level regulation of PA metabolism led to the accumulation of putrescine (Put). Arginine decarboxylase (ADC) had an increased expression trend under potassium deficiency, whereas spermidine synthase (SPDS) expression decreased. Generally, free spermidine (Spd) and spermine (Spm)/ thermospermine (t-Spm) contents were kept relatively stable, mostly by the downregulation of polyamine oxidase (PAO) expression. The low potassium contents in the culture medium decreased the potassium content of the cells, which inhibited cell mass growth, but did not affect cell viability. The reduced growth was probably caused by repressed metabolic activity and cell division, whereas there were no signs of H₂O₂-induced oxidative stress or increased cell death. The low intracellular content of K⁺ decreased the content of Na⁺. The decrease in the pH of the culture medium indicated that H⁺ ions were pumped out of the cells. Altogether, our findings emphasize the specific role(s) of Put under potassium deficiency and strict developmental regulation of PA metabolism in Scots pine.

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Series: Cells
ISSN: 2073-4409
ISSN-E: 2073-4409
ISSN-L: 2073-4409
Volume: 10
Issue: 5
Article number: 1244
DOI: 10.3390/cells10051244
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Copyright information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (