Öztürk-Öncel, M. Ö., Erkoc-Biradli, F. Z., Rasier, R., Marcali, M., Elbuken, C., & Garipcan, B. (2021). Rose petal topography mimicked poly(dimethylsiloxane) substrates for enhanced corneal endothelial cell behavior. Materials Science and Engineering: C, 126, 112147. https://doi.org/10.1016/j.msec.2021.112147
Rose petal topography mimicked poly(dimethylsiloxane) substrates for enhanced corneal endothelial cell behavior
|Author:||Öztürk-Öncel, M. Özgen1; Erkoc-Biradli, Fatma Zehra1; Rasier, Rıfat2;|
1Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey
2Department of Ophthalmology, Demiroglu Bilim University, Istanbul, Turkey
3UNAM-National Nanotechnology Research Center, Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey
4Faculty of Biochemistry and Molecular Medicine, Faculty of Medicine, University of Oulu, 90014 Oulu, Finland
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2021092046672
|Publish Date:|| 2023-04-30
Low proliferation capacity of corneal endothelial cells (CECs) and worldwide limitations in transplantable donor tissues reveal the critical need of a robust approach for in vitro CEC growth. However, preservation of CEC-specific phenotype with increased proliferation has been a great challenge. Here we offer a biomimetic cell substrate design, by optimizing mechanical, topographical and biochemical characteristics of materials with CEC microenvironment. We showed the surprising similarity between topographical features of white rose petals and corneal endothelium due to hexagonal cell shapes and physiologically relevant cell density (≈ 2000 cells/mm²). Polydimethylsiloxane (PDMS) substrates with replica of white rose petal topography and cornea-friendly Young’s modulus (211.85 ± 74.9 kPa) were functionalized with two of the important corneal extracellular matrix (ECM) components, collagen IV (COL 4) and hyaluronic acid (HA). White rose petal patterned and COL 4 modified PDMS with optimized stiffness provided enhanced bovine CEC response with higher density monolayers and increased phenotypic marker expression. This biomimetic approach demonstrates a successful platform to improve in vitro cell substrate properties of PDMS for corneal applications, suggesting an alternative environment for CEC-based therapies, drug toxicity investigations, microfluidics and organ-on-chip applications.
Materials science & engineering. C, Materials for biological applications
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1182 Biochemistry, cell and molecular biology
This study was supported by Bogazici University Research Fund by Grant Numbers 11501 and 6701.
© 2021 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.