University of Oulu

Öztürk-Öncel, M. Ö., Erkoc-Biradli, F. Z., Rasier, R., Marcali, M., Elbuken, C., & Garipcan, B. (2021). Rose petal topography mimicked poly(dimethylsiloxane) substrates for enhanced corneal endothelial cell behavior. Materials Science and Engineering: C, 126, 112147. https://doi.org/10.1016/j.msec.2021.112147

Rose petal topography mimicked poly(dimethylsiloxane) substrates for enhanced corneal endothelial cell behavior

Saved in:
Author: Öztürk-Öncel, M. Özgen1; Erkoc-Biradli, Fatma Zehra1; Rasier, Rıfat2;
Organizations: 1Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey
2Department of Ophthalmology, Demiroglu Bilim University, Istanbul, Turkey
3UNAM-National Nanotechnology Research Center, Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey
4Faculty of Biochemistry and Molecular Medicine, Faculty of Medicine, University of Oulu, 90014 Oulu, Finland
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2021092046672
Language: English
Published: Elsevier, 2021
Publish Date: 2023-04-30
Description:

Abstract

Low proliferation capacity of corneal endothelial cells (CECs) and worldwide limitations in transplantable donor tissues reveal the critical need of a robust approach for in vitro CEC growth. However, preservation of CEC-specific phenotype with increased proliferation has been a great challenge. Here we offer a biomimetic cell substrate design, by optimizing mechanical, topographical and biochemical characteristics of materials with CEC microenvironment. We showed the surprising similarity between topographical features of white rose petals and corneal endothelium due to hexagonal cell shapes and physiologically relevant cell density (≈ 2000 cells/mm²). Polydimethylsiloxane (PDMS) substrates with replica of white rose petal topography and cornea-friendly Young’s modulus (211.85 ± 74.9 kPa) were functionalized with two of the important corneal extracellular matrix (ECM) components, collagen IV (COL 4) and hyaluronic acid (HA). White rose petal patterned and COL 4 modified PDMS with optimized stiffness provided enhanced bovine CEC response with higher density monolayers and increased phenotypic marker expression. This biomimetic approach demonstrates a successful platform to improve in vitro cell substrate properties of PDMS for corneal applications, suggesting an alternative environment for CEC-based therapies, drug toxicity investigations, microfluidics and organ-on-chip applications.

see all

Series: Materials science & engineering. C, Materials for biological applications
ISSN: 0928-4931
ISSN-E: 1873-0191
ISSN-L: 0928-4931
Volume: 126
Article number: 112147
DOI: 10.1016/j.msec.2021.112147
OADOI: https://oadoi.org/10.1016/j.msec.2021.112147
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Subjects:
Funding: This study was supported by Bogazici University Research Fund by Grant Numbers 11501 and 6701.
Copyright information: © 2021 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/