University of Oulu

Ruixue Zhu, Tatiana Avsievich, Alexey Popov, Alexander Bykov, and Igor Meglinski "In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 1179809 (1 August 2021);

In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

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Author: Zhu, Ruixue1; Avsievich, Tatiana1; Popov, Alexey2;
Organizations: 1Optoelectronics and Measurement Techniques Laboratory, University of Oulu, 90570 Oulu, Finland
2VTT Technical Research Centre of Finland, Kaitoväylä 1, 90590, Oulu, Finland
3College of Engineering and Physical Sciences, Aston University, Birmingham, B4 7ET, UK
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.1 MB)
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Language: English
Published: SPIE, 2021
Publish Date: 2021-09-27


The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

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Series: Proceedings of SPIE
ISSN: 0277-786X
ISSN-E: 1996-756X
ISSN-L: 0277-786X
ISBN: 978-1510-6443-5-9
ISBN Print: 978-151-06443-4-2
Pages: 1 - 7
DOI: 10.1117/12.2595118
Host publication: Optical Trapping and Optical Micromanipulation XVIII, 1–5 August 2021. San Diego, USA
Host publication editor: Dholakia, Kishan
Spalding, Gabriel C.
Conference: Optical Trapping and Optical Micromanipulation
Type of Publication: A4 Article in conference proceedings
Field of Science: 222 Other engineering and technologies
Funding: This work was partially funded by the China Scholarship Council (CSC No.201706410089, R.Z.). R.Z. acknowledges X.Y. Su (School of Science, Beijing Jiaotong University, Beijing, China) for technical help in laser irradiation source selection. The authors also acknowledge the contribution of Russian Science Foundation (Projects: 19-72-30012 and 20-64-46003). I.M. acknowledges partial support from the Academy of Finland (project 325,097).
Academy of Finland Grant Number: 325097
Detailed Information: 325097 (Academy of Finland Funding decision)
Copyright information: © (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. Archived here with the kind permission by the Publisher.