University of Oulu

Andrey Y. Vinokurov, Olga A. Stelmashuk, Polina A. Ukolova, Evgeny A. Zherebtsov, Andrey Y. Abramov, Brain region specificity in reactive oxygen species production and maintenance of redox balance, Free Radical Biology and Medicine, Volume 174, 2021, Pages 195-201, ISSN 0891-5849, https://doi.org/10.1016/j.freeradbiomed.2021.08.014

Brain region specificity in reactive oxygen species production and maintenance of redox balance

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Author: Vinokurov, Andrey Y.1; Stelmashuk, Olga A.1; Ukolova, Polina A.1;
Organizations: 1Cell Physiology and Pathology Laboratory, Orel State University, Orel, 302026, Russia
2Optoelectronics and Measurement Techniques Laboratory, University of Oulu, Oulu, 90014, Finland
3Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, Queen Square, London WC1N 3BG, UK
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2021100449276
Language: English
Published: Elsevier, 2021
Publish Date: 2023-08-13
Description:

Abstract

The brain produces various reactive oxygen species in enzymatic and non-enzymatic reactions as a by-product of metabolism and/or for redox signaling. Effective antioxidant system in the brain cells maintains redox balance. However, neurons and glia from some brain regions are more vulnerable to oxidative stress in ischemia/reperfusion, epilepsy, and neurodegenerative disorders than the rest of the brain. Using fluorescent indicators in live cell imaging and confocal microscopy, we have measured the rate of cytosolic and mitochondrial reactive oxygen species production, lipid peroxidation, and glutathione levels in cortex, hippocampus, midbrain, brain stem and cerebellum in acute slices of rat brain. We have found that the basal rate of ROS production is at its highest in brain stem and cerebellum, and that it is mainly generated by glial cells. Activation of neurons and glia by glutamate and ATP led to maximal rates of ROS production in the midbrain compared to the rest of the brain. Mitochondrial ROS had only minor implication to the total ROS production with maximal values in the cortex and minimal in the midbrain. The basal rate of lipid peroxidation was higher in the midbrain and hippocampus, while the GSH level was similar in most brain regions with the lowest level in the midbrain. Thus, the rate of ROS production, lipid peroxidation and the level of GSH vary across brain regions.

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Series: Free radical biology & medicine
ISSN: 0891-5849
ISSN-E: 1873-4596
ISSN-L: 0891-5849
Volume: 174
Pages: 195 - 201
DOI: 10.1016/j.freeradbiomed.2021.08.014
OADOI: https://oadoi.org/10.1016/j.freeradbiomed.2021.08.014
Type of Publication: A1 Journal article – refereed
Field of Science: 217 Medical engineering
1182 Biochemistry, cell and molecular biology
Subjects:
GSH
Funding: This work was supported by the grant of the Russian Federation Government no. 075-15-2019-1877.
Copyright information: © 2021 Elsevier Inc. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
  https://creativecommons.org/licenses/by-nc-nd/4.0/