University of Oulu

Isiksacan, Z., Serhatlioglu, M., & Elbuken, C. (2020). In vitro analysis of multiple blood flow determinants using red blood cell dynamics under oscillatory flow. The Analyst, 145(18), 5996–6005.

In vitro analysis of multiple blood flow determinants using red blood cell dynamics under oscillatory flow

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Author: Isiksacan, Ziya1; Serhatlioglu, Murat1; Elbuken, Caglar1,2
Organizations: 1UNAM – National Nanotechnology Research Center and Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey
2Faculty of Biochemistry and Molecular Medicine, Faculty of Medicine, University of Oulu, 90014 Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.3 MB)
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Language: English
Published: Royal Society of Chemistry, 2020
Publish Date: 2021-10-06


The flow behavior of blood is determined mainly by red blood cell (RBC) deformation and aggregation as well as blood viscoelasticity. These intricately interdependent parameters should be monitored by healthcare providers to understand all aspects of circulatory flow dynamics under numerous cases including cardiovascular and infectious diseases. Current medical instruments and microfluidic systems lack the ability to quantify these parameters all at once and in physiologically relevant flow conditions. This work presents a handheld platform and a measurement method for quantitative analysis of multiple of these parameters from 50 μl undiluted blood inside a miniaturized channel. The assay is based on an optical transmission analysis of collective RBC deformation and aggregation under near-infrared illumination during a 1 s damped oscillatory flow and at stasis, respectively. Measurements with blood of different hemo-rheological properties demonstrate that the presented approach holds a potential for initiating simultaneous and routine on-chip blood flow analysis even in resource-poor settings.

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Series: Analyst
ISSN: 0003-2654
ISSN-E: 1364-5528
ISSN-L: 0003-2654
Volume: 145
Issue: 18
Pages: 5996 - 6005
DOI: 10.1039/D0AN00604A
Type of Publication: A1 Journal article – refereed
Field of Science: 318 Medical biotechnology
Funding: Dr Elbuken acknowledges support from The Science Academy, Turkey, through the Young Scientist Award Program. Ziya Isiksacan is supported by ASELSAN Graduate Scholarship for Turkish Academicians. The authors acknowledge support from The Scientific and Technologic Research Council of Turkey (TUBITAK project no. 213S127).
Copyright information: © 2020 The Authors and the Royal Society of Chemistry. The final authenticated version is available online at