Presence of distinctive microbiome in the first-pass meconium of newborn infants
|Author:||Turunen, Jenni1,2; Tejesvi, Mysore V.2,3; Paalanne, Niko1,4;|
1Medical Research Center and PEDEGO Research Unit, University of Oulu, Oulu, Finland
2Biocenter Oulu, University of Oulu, Oulu, Finland
3Ecology and Genetics, Faculty of Science, University of Oulu, Oulu, Finland
4Department of Pediatrics and Adolescent Medicine, Oulu University Hospital, Oulu, Finland
5Cancer and Translational Medicine Research Unit, University of Oulu, Oulu, Finland
6Medical Research Center Oulu, University of Oulu, Oulu, Finland
7Department of Pathology, Oulu University Hospital, Oulu, Finland
|Online Access:||PDF Full Text (PDF, 3.2 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2021101450978
|Publish Date:|| 2021-10-14
We critically evaluated the fetal microbiome concept in 44 neonates with placenta, amniotic fluid, and first-pass meconium samples. Placental histology showed no signs of inflammation. Meconium samples were more often bacterial culture positive after vaginal delivery. In next-generation sequencing of the bacterial 16S gene, before and after removal of extracellular and PCR contaminant DNA, the median number of reads was low in placenta (48) and amniotic fluid (46) and high in meconium samples (14,556 C-section, 24,860 vaginal). In electron microscopy, meconium samples showed extracellular vesicles. Utilizing the analysis of composition of microbiomes (ANCOM) against water, meconium samples had a higher relative abundance of Firmicutes, Lactobacillus, Streptococcus, and Escherichia-Shigella. Our results did not support the existence of the placenta and amniotic fluid microbiota in healthy pregnancies. The first-pass meconium samples, formed in utero, appeared to harbor a microbiome that may be explained by perinatal colonization or intrauterine colonization via bacterial extracellular vesicles.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
3123 Gynaecology and paediatrics
1183 Plant biology, microbiology, virology
JR wishes to thank the Academy of Finland for Grants 299749 and 328768. TT would like to thank the Academy of Finland for Clinical Research Grant 2018-2022 and Pediatric Research Foundation Grant 2019-2022.
|Academy of Finland Grant Number:||
299749 (Academy of Finland Funding decision)
328768 (Academy of Finland Funding decision)
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