University of Oulu

Rantala, S., Kaseva, J., Nukari, A., Laamanen, J., Veteläinen, M., Häggman, H., & Karhu, S. (2021). Successful Cryopreservation of Dormant Buds of Blackcurrant (Ribes nigrum L.) by Using Greenhouse-Grown Plants and In Vitro Recovery. Plants, 10(7), 1414. doi:10.3390/plants10071414

Successful cryopreservation of dormant buds of blackcurrant (Ribes nigrum L.) by using greenhouse-grown plants and in vitro recovery

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Author: Rantala, Saija1,2; Kaseva, Janne3; Nukari, Anna4;
Organizations: 1Natural Resources Institute Finland (Luke), Production Systems, Survontie 9 A, FI-40500 Jyväskylä, Finland
2Ecology and Genetics Unit, Faculty of Science, University of Oulu, P.O. Box 3000, FI-90014 Oulu, Finland
3Natural Resources Institute Finland (Luke), Natural Resources, Tietotie 4, FI-31600 Jokioinen, Finland
4Natural Resources Institute Finland (Luke), Production Systems, Latokartanonkaari 9, FI-00790 Helsinki, Finland
5Natural Resources Institute Finland (Luke), Natural Resources, Survontie 9 A, FI-40500 Jyväskylä, Finland
6Boreal Plant Breeding Ltd., Myllytie 10, FI-31600 Jokioinen, Finland
7Natural Resources Institute Finland (Luke), Production Systems, Itäinen Pitkäkatu 4 A, FI-20520 Turku, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.4 MB)
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Language: English
Published: Multidisciplinary Digital Publishing Institute, 2021
Publish Date: 2021-10-20


The cryopreservation of dormant buds can be a feasible method for preserving germplasm of cold-tolerant woody plants. In the present study, we evaluated the effects of pre-desiccation, thawing method, and the rehydration of bud sections on the post-cryopreservation recovery of dormant blackcurrant buds in vitro. The estimated recovery of small- and medium-sized buds was 80.1 and 62.7% respectively for desiccated buds and 67.8 and 72.3% respectively for non-desiccated buds. The pre-desiccation of bud sections enhanced the number of the shoots regenerated from vegetative buds (2.3 vs. 4.7). The estimated recovery of fast-thawed buds was better after 14-day than after 7-day rehydration (85 vs. 59%). In slowly thawed buds the difference between 14-day and 7-day rehydration was not significant (73 vs. 62%). The estimated recovery of vegetative and flower buds was 77.7 and 41.1% respectively after 7-day rehydration, and 95.2 and 43.6% respectively after a 14-day rehydration period. The rehydration of bud sections was not necessary for the in vitro recovery of non-desiccated, fast-thawed buds. Of the 23 blackcurrant cultivars cryopreserved using non-desiccated dormant buds collected from a greenhouse, the estimated recovery of 22 cultivars ranged between 42 and 90%.

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Series: Plants
ISSN: 2223-7747
ISSN-E: 2223-7747
ISSN-L: 2223-7747
Volume: 10
Issue: 7
Article number: 1414
DOI: 10.3390/plants10071414
Type of Publication: A1 Journal article – refereed
Field of Science: 1183 Plant biology, microbiology, virology
Funding: This research was partly funded by the European Commission, Directorate-General for Agriculture and Rural Development, under Council Regulation (EC) No. 870/2004 through Action 071 AGRI GEN RES 870/2004 (RIBESCO), by the Ministry of Agriculture and Forestry, and by the foundations of Maiju and Yrjö Rikalan Puutarhasäätiö and Oiva Kuusisto Säätiö.
Copyright information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (