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Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
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| Author: | Vergauwen, Glenn1,2,3; Tulkens, Joeri1,2; Pinheiro, Cláudio1,2; |
| Organizations: |
1Department of Human Structure and Repair, Laboratory of Experimental Cancer Research, Ghent University, Ghent, Belgium 2Cancer Research Institute Ghent, Ghent, Belgium 3Department of Gynecology, Ghent University Hospital, Ghent, Belgium
4Department of Biomolecular Medicine, OncoRNALab, Ghent University, Ghent, Belgium
5Department of Internal Medicine and Pediatrics, HIV Cure Research Center, Ghent University Hospital, Ghent, Belgium 6VIB Center for Medical Biotechnology, Ghent, Belgium 7Department of Biomolecular Medicine, Ghent University, Ghent, Belgium 8VIB Proteomics Core, Ghent, Belgium 9Biocenter Oulu, University of Oulu, Oulu, Finland 10Department of Medical Oncology, Ghent University Hospital, Ghent, Belgium |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 9 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe2021110453740 |
| Language: | English |
| Published: |
Informa,
2021
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| Publish Date: | 2021-11-04 |
| Description: |
AbstractSeparating extracellular vesicles (EV) from blood plasma is challenging and complicates their biological understanding and biomarker development. In this study, we fractionate blood plasma by combining size-exclusion chromatography (SEC) and OptiPrep density gradient centrifugation to study clinical context-dependent and time-dependent variations in the biomolecular landscape of systemically circulating EV. Using pooled blood plasma samples from breast cancer patients, we first demonstrate the technical repeatability of blood plasma fractionation. Using serial blood plasma samples from HIV and ovarian cancer patients (n = 10) we next show that EV carry a clinical context-dependent and/or time-dependent protein and small RNA composition, including miRNA and tRNA. In addition, differential analysis of blood plasma fractions provides a catalogue of putative proteins not associated with systemically circulating EV. In conclusion, the implementation of blood plasma fractionation allows to advance the biological understanding and biomarker development of systemically circulating EV. see all
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| Series: |
Journal of extracellular vesicles |
| ISSN: | 2001-3078 |
| ISSN-E: | 2001-3078 |
| ISSN-L: | 2001-3078 |
| Volume: | 10 |
| Issue: | 10 |
| Article number: | e12122 |
| DOI: | 10.1002/jev2.12122 |
| OADOI: | https://oadoi.org/10.1002/jev2.12122 |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
3111 Biomedicine |
| Subjects: | |
| Funding: |
This work was supported by Concerted Research Actions from Ghent University, the Fund for Scientific Spearheads of Ghent University Hospital, the National Cancer Plan, the Research Foundation-Flanders and Kom op tegen Kanker (Stand up to Cancer) and European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No [722148]. |
| Copyright information: |
© 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
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https://creativecommons.org/licenses/by/4.0/ |