University of Oulu

Tatiana Avsievich, Yana Tarakanchikova, Alexey Popov, Artashes Karmenyan, Alexander Bykov, Igor Meglinski, "Combined use of optical tweezers and scanning electron microscopy to reveal influence of nanoparticles on red blood cells interactions," Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110750F (22 July 2019); https://doi.org/10.1117/12.2526730

Combined use of optical tweezers and scanning electron microscopy to reveal influence of nanoparticles on red blood cells interactions

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Author: Avsievich, Tatiana1; Tarakanchikova, Yana1; Popov, Alexey1;
Organizations: 1Optoelectronics and Measurement Techniques, University of Oulu, Oulu, FI-90014, Finland
2Infrared Laboratory, Department of Physics, National Dong Hwa University, Hualien, ROC, Taiwan
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.9 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021110553921
Language: English
Published: SPIE, 2019
Publish Date: 2021-11-05
Description:

Abstract

As a promising drug delivery system, itself or coupled with red blood cells (RBC), nanoparticles (NP) should be studied in frames of their interaction at the cellular level. Experiments were performed on RBC in autologous blood plasma incubated with different NP — TiO₂, ZnO, nanodiamonds and polymeric nanocapsules. RBC aggregates formation in RBC suspension was observed with conventional microscopy, while quantitative interaction force measurements between individual RBC was assessed with optical tweezers. Scanning electron microscopy (SEM) imaging demonstrated NP localization and RBC membrane modifications upon binding with NP. Among tested NP, nanodiamonds caused increasing the size of aggregates in RBC suspensions, RBC interaction force increase and strong membrane surface modifications, comparing to other tested NP and control sample. Nanocapsules do not cause any adverse effects on RBC properties, confirming biocompatibility and applicability for drug delivery purposes. Optical tweezers combined with SEM imaging serves as fast informative assessment of NP effects on RBC.

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Series: Proceedings of SPIE
ISSN: 0277-786X
ISSN-E: 1996-756X
ISSN-L: 0277-786X
ISBN: 978-1-5106-2843-4
Issue: 11075
Article number: 11075F
DOI: 10.1117/12.2526730
OADOI: https://oadoi.org/10.1117/12.2526730
Conference: European Conferences on Biomedical Optics, 2019, Munich, Germany
Field of Science: 213 Electronic, automation and communications engineering, electronics
217 Medical engineering
Subjects:
Copyright information: © 2019 Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited.