University of Oulu

D’Souza, R., Kazi, S., & Harshal, A. (2020). Isolation and characterization of a klebsiella strain from smokeless tobacco consumers. International Journal of Scientific & Technology Research, 9(4), 1097-1100.

Isolation and characterization of a Klebsiella strain from smokeless tobacco consumers

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Author: D’Souza, Racheal1; Kazi, Samina2; Harshal, Anupma3
Organizations: 1Boston, USA
2University of Oulu, Finland
3Indian Institute of Science, Education and Research, India
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021111655669
Language: English
Published: Amazedia Solutions, 2020
Publish Date: 2021-11-16
Description:

Abstract

Organisms that reside in the oral cavity become an important aspect of health and disease. Disruption of the oral health and hygiene has been attributed to the presence of abnormal microflora. Consumption of tobacco has been considered to be a leading cause for the presence of abnormal oral microflora, in turn disrupting the oral health. In this study, 45 respondents were targeted from South Mumbai who consumed tobacco in smokeless forms. Klebsiella pneumoniae strain was isolated from oral swabs of smokeless tobacco consumers and compared against controls. The strain was characterized using phenotypic and genotypic approaches. The presence of Klebsiella was reported in 24 of the 45 samples. Sequence comparison of 16S rRNA gene and phylogenetic analysis confirmed the presence of Klebsiella strain. The sequence obtained was submitted to the NCBI Gene bank that has been granted an accession number- KM186520. During the course of our study we observed Klebsiella pneumoniae to exhibit green fluorescent colonies under UV spectroscopy. λmax of the fluorescent chromophore was estimated to be 220 nm with OD of 1.785. Our analysis provides evidence that Klebsiella pneumoniae in the oral micro flora of the tobacco consumers exhibits a novel phenomenon of fluorescence.

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Series: International journal of scientific & technological research
ISSN: 2277-8616
ISSN-E: 2277-8616
ISSN-L: 2277-8616
Volume: 9
Issue: 4
Pages: 1097 - 1100
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Subjects:
Copyright information: © 2020 IJSTR. The Journal retains the copyrights of all material published in the issue. However, reproduction of the published material in the part or total in any form is permitted with due acknowledgement of the source as per ethical norms.