University of Oulu

Kipkoech, C.; Kinyuru, J.N.; Imathiu, S.; Meyer-Rochow, V.B.; Roos, N. In Vitro Study of Cricket Chitosan’s Potential as a Prebiotic and a Promoter of Probiotic Microorganisms to Control Pathogenic Bacteria in the Human Gut. Foods 2021, 10, 2310. https://doi.org/10.3390/foods10102310

In vitro study of cricket chitosan’s potential as a prebiotic and a promoter of probiotic microorganisms to control pathogenic bacteria in the human gut

Saved in:
Author: Kipkoech, Carolyne1,2; Kinyuru, John N.1; Imathiu, Samuel1;
Organizations: 1Department of Food Science and Technology, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya
2Federal Institute of Risk assessment, D-12107 Berlin, Germany
3Department of Plant Medicals (Agricultural Science and Technology), Andong National University, Andong 36729, Korea
4Department of Genetics and Ecology, Oulu University, SF-90140 Oulu, Finland
5Department of Nutrition, Exercise and Sports, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg, Denmark
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.7 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021111956064
Language: English
Published: Multidisciplinary Digital Publishing Institute, 2021
Publish Date: 2021-11-19
Description:

Abstract

In this study, cricket chitosan was used as a prebiotic. Lactobacillus fermentum, Lactobacillus acidophilus, and Bifidobacterium adolescentis were identified as probiotic bacteria. Cricket chitin was deacetylated to chitosan and added to either De Man Rogosa and Sharpe or Salmonella/Shigella bacterial growth media at the rates of 1%, 5%, 10%, or 20% to obtain chitosan-supplemented media. The growth of the probiotic bacteria was monitored on chitosan-supplemented media after 6, 12, 24, and 48 h upon incubation at 37 °C. Growth of Salmonella typhi in the presence of probiotic bacteria in chitosan-supplemented media was evaluated under similar conditions to those of the growth of probiotic bacteria by measuring growth inhibition zones (in mm) around the bacterial colonies. All chitosan concentrations significantly increased the populations of probiotic bacteria and decreased the populations of pathogenic bacteria. During growth, there was a significant pH change in the media with all probiotic bacteria. Inhibition zones from probiotic bacteria growth supernatant against Salmonella typhi were most apparent at 16 mm and statistically significant in connection with a 10% chitosan concentration. This study suggests cricket-derived chitosan can function as a prebiotic, with an ability to eliminate pathogenic bacteria in the presence of probiotic bacteria.

see all

Series: Foods
ISSN: 2304-8158
ISSN-E: 2304-8158
ISSN-L: 2304-8158
Volume: 10
Issue: 10
Article number: 2310
DOI: 10.3390/foods10102310
OADOI: https://oadoi.org/10.3390/foods10102310
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
Subjects:
Funding: Financial support for CK came from DANIDA funded by the Ministry of Foreign Affairs Denmark (2013–2018) under the funding no 13-06KU GREEiNSECT project. VBM-R was supported by a grant from the National Research Foundation of Korea (NRF-2018R1A6A1A03024862) to Prof. Chuleui Jung of Andong National University’s Basic Science Research Program. Ethical approval for this study was issued by the Director of Mount Kenya University through Dr Francis W. Muregi of the university’s Ethics Review Committee (ERC) on 09/01/2017 under the number AG422-4492/2015 to Principal Investigator Carolyne Kipkoech.
Copyright information: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
  https://creativecommons.org/licenses/by/4.0/