Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference |
|
Author: | Bayoumy, Sherif1; Martiskainen, Iida1; Heikkilä, Taina1; |
Organizations: |
1Department of Biotechnology, University of Turku, Turku, Finland 2Cancer Research and Translational Medicine Research Unit, Department of Clinical Chemistry, University of Oulu, Kajaanintie 50, 90220, Oulu, Finland 3The Joint Municipal Service Provider of Northern Finland Laboratory Centre (NordLab), Kiviharjuntie 11, 90220, Oulu, Finland |
Format: | article |
Version: | published version |
Access: | open |
Online Access: | PDF Full Text (PDF, 1.5 MB) |
Persistent link: | http://urn.fi/urn:nbn:fi-fe2021112356442 |
Language: | English |
Published: |
Springer Nature,
2021
|
Publish Date: | 2021-11-23 |
Description: |
AbstractMeasurement of cardiac troponin I (cTnI) should be feasible for point-of-care testing (POCT) to diagnose acute myocardial infarction (AMI). Lateral flow immunoassays (LFIAs) have been long implemented in POCT and clinical settings. However, sensitivity, matrix effect and quantitation in lateral flow immunoassays (LFIAs) have been major limiting factors. The performance of LFIAs can be improved with upconverting nanoparticle (UCNP) reporters. Here we report a new methodological approach to quantify cTnI using UCNP-LFIA technology with minimized plasma interference. The performance of the developed UCNP-LFIA was evaluated using clinical plasma samples (n = 262). The developed UCNP-LFIA was compared to two reference assays, the Siemens Advia Centaur assay and an in-house well-based cTnI assay. By introducing an anti-IgM scrub line and dried EDTA in the LFIA strip, the detection of cTnI in plasma samples was fully recovered. The UCNP-LFIA was able to quantify cTnI concentrations in patient samples within the range of 30–10,000 ng/L. The LoB and LoD of the UCNP-LFIA were 8.4 ng/L and 30 ng/L. The method comparisons showed good correlation (Spearman’s correlation 0.956 and 0.949, p < 0.0001). The developed UCNP-LFIA had LoD suitable for ruling in AMI in patients with elevated cTnI levels and was able to quantify cTnI concentrations in patient samples. The technology has potential to provide simple and rapid assay for POCT in ED setting. see all
|
Series: |
Scientific reports |
ISSN: | 2045-2322 |
ISSN-E: | 2045-2322 |
ISSN-L: | 2045-2322 |
Volume: | 11 |
Issue: | 1 |
Article number: | 18698 |
DOI: | 10.1038/s41598-021-98199-y |
OADOI: | https://oadoi.org/10.1038/s41598-021-98199-y |
Type of Publication: |
A1 Journal article – refereed |
Field of Science: |
3122 Cancers |
Subjects: | |
Funding: |
This work was partly carried out within the project titled ‘Upconverting Nanoparticles for High Performance Applications in Lateral Flow and Microfluidic Platforms (UP-POC) and supported by the Business Finland foundation, Finland; [Grant Number 2208/31/2016]. We gratefully acknowledge the UP-POC project consortium for the co-operation and resources invested during the project. We would also like to acknowledge Kaivogen Oy for providing the upconverting nanoparticles needed during the work. We are also grateful to Hytest for providing antibodies and expertise required for this work. |
Copyright information: |
© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
https://creativecommons.org/licenses/by/4.0/ |