University of Oulu

Samoylenko, A., Kögler, M., Zhyvolozhnyi, A. et al. Time-gated Raman spectroscopy and proteomics analyses of hypoxic and normoxic renal carcinoma extracellular vesicles. Sci Rep 11, 19594 (2021). https://doi.org/10.1038/s41598-021-99004-6

Time-gated Raman spectroscopy and proteomics analyses of hypoxic and normoxic renal carcinoma extracellular vesicles

Saved in:
Author: Samoylenko, Anatoliy1; Kögler, Martin2; Zhyvolozhnyi, Artem1;
Organizations: 1Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu and Kvantum Institute, 90014, Oulu, Finland
2VTT Technical Research Centre of Finland, 90570, Oulu, Finland
3Institute of Photonics, University of Eastern Finland, 80101, Joensuu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.8 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021112456715
Language: English
Published: Springer Nature, 2021
Publish Date: 2021-11-24
Description:

Abstract

Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles involved in cell–cell communication, but the technologies to characterize EVs are still limited. Hypoxia is a typical condition in solid tumors, and cancer-derived EVs support tumor growth and invasion of tissues by tumor cells. We found that exposure of renal adenocarcinoma cells to hypoxia induced EV secretion and led to notable changes in the EV protein cargo in comparison to normoxia. Proteomics analysis showed overrepresentation of proteins involved in adhesion, such as integrins, in hypoxic EV samples. We further assessed the efficacy of time-gated Raman spectroscopy (TG-RS) and surface-enhanced time-gated Raman spectroscopy (TG-SERS) to characterize EVs. While the conventional continuous wave excitation Raman spectroscopy did not provide a notable signal, prominent signals were obtained with the TG-RS that were further enhanced in the TG-SERS. The Raman signal showed characteristic changes in the amide regions due to alteration in the chemical bonds of the EV proteins. The results illustrate that the TG-RS and the TG-SERS are promising label free technologies to study cellular impact of external stimuli, such as oxygen deficiency, on EV production, as well as differences arising from distinct EV purification protocols.

see all

Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 11
Issue: 1
Article number: 19594
DOI: 10.1038/s41598-021-99004-6
OADOI: https://oadoi.org/10.1038/s41598-021-99004-6
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
3111 Biomedicine
Subjects:
Funding: The work is part of the Academy of Finland (AF) Flagship Programs: Photonics Research and Innovation (PREIN) with decisions 320168 (M.K., J.H) and 320166 (S.A., M.R) and Gene, Cell and Nano Therapy Competence Cluster for the Treatment of Chronic Diseases (GeneCellNano) with decisions 337431 (S.V., A.S., G.B.) and 315030 (S.V.). The work by Univ. Oulu and VTT groups was partially funded by FET Open MindGap-project (EU Grant agreement ID: 829040). The study was supported by the grants from Cancer Foundation of Finland to S.V. (2017 and 2018), Business Finland Future of Diagnostics—FUDIS, and EDUFI (Finnish National Agency for Education) Fellowships.
Academy of Finland Grant Number: 337431
Detailed Information: 337431 (Academy of Finland Funding decision)
Copyright information: © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
  https://creativecommons.org/licenses/by/4.0/