University of Oulu

Jaafar, A., Mahmood, M. H., Holomb, R., Himics, L., Váczi, T., Sdobnov, A. Y., Tuchin, V. V., & Veres, M. (2021) Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution. Innovative Optical Health Sciences 14(5), 2142003,

Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution

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Author: Jaafar, Ali1,2,3; Mahmood, Malik H.1,2,4; Holomb, Roman1,5;
Organizations: 1Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, P.O. Box 49, H-1121 Budapest, Hungary
2Institute of Physics, University of Szeged, Dom ter 9, H-6720 Szeged, Hungary
3Ministry of Higher Education and Scientific Research, Baghdad 10065, Iraq
4Physiology Department, College of Medicine, University of Misan, Al-Amarah, Misan 62001, Iraq
5Uzhhorod National University, Uzhhorod 88015, Transcarpathia, Ukraine
6Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., Saratov 410012, Russia
7Optoelectronics and Measurement Techniques Laboratory, University of Oulu, 90570 Oulu, Finland
8Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the Russian Academy of Sciences, 24 Rabochaya, Saratov 410028, Russia
9Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, 36 Lenin Avenue, Tomsk 634050, Russia
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.4 MB)
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Language: English
Published: World Scientific, 2021
Publish Date: 2021-12-16


Confocal Raman microspectroscopy (CRM) with 633- and 785-nm excitation wavelengths combined with optical clearing (OC) technique was used for ex-vivo study of porcine skin in the Raman fingerprint region. The optical clearing has been performed on the skin samples by applying a mixture of glycerol and distilled water and a mixture of glycerol, distilled water and chemical penetration enhancer dimethyl sulfoxide (DMSO) during 30min and 60min of treatment. It was shown that the combined use of the optical clearing technique and CRM at 633nm allowed one to preserve the high probing depth, signal-to-noise ratio and spectral resolution simultaneously. Comparing the effect of different optical clearing agents on porcine skin showed that an optical clearing agent containing chemical penetration enhancer provides higher optical clearing efficiency. Also, an increase in treatment time allows to improve the optical clearing efficiency of both optical clearing agents. As a result of optical clearing, the detection of the amide-III spectral region indicating well-distinguishable structural differences between the type-I and type-IV collagens has been improved.

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Series: Journal of innovative optical health sciences
ISSN: 1793-5458
ISSN-E: 1793-7205
ISSN-L: 1793-5458
Volume: 14
Issue: 5
Article number: 2142003
DOI: 10.1142/S1793545821420037
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: This work was done under a scholarship of the Stipendium Hungaricum Scholarship Programme. This work was supported by the VEKOP-2.3.2-16-2016-00011 Grant, which is co-financed by the European Union and European Social Fund. Valery V. Tuchin was supported by RFBR Grant 18-52-16025 and the Grant of the Government of the Russian Federation (Registration No. 2020-220-08-2389).
Copyright information: © The Author(s). This is an Open Access article. It is distributed under the terms of the Creative Commons Attribution 4.0 (CC-BY) License. Further distribution of this work is permitted, provided the original work is properly cited.