University of Oulu

Marttila, E., Rusanen, P., Uittamo, J., Salaspuro, M., Rautemaa-Richardson, R., & Salo, T. (2021). Expression of p53 is associated with microbial acetaldehyde production in oralsquamous cell carcinoma. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 131(5), 527–533. https://doi.org/10.1016/j.oooo.2020.11.015

Expression of p53 is associated with microbial acetaldehyde production in oralsquamous cell carcinoma

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Author: Marttila, Emilia1; Rusanen, Peter2; Uittamo, Johanna1,3;
Organizations: 1Department of Oral and Maxillofacial Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland
2Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
3Research Unit on Acetaldehyde and Cancer, University of Helsinki, Helsinki, Finland
4Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, NIHR Manchester Biomedical Research Centre (BRC) at the Manchester Academic Health Science Centre, The University of Manchester, Manchester, UK
5Manchester University NHS Foundation Trust, Wythenshawe Hospital, Manchester, UK
6HUSLAB, Department of Pathology, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
7Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, and University of Helsinki, Helsinki, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2021122162815
Language: English
Published: Elsevier, 2021
Publish Date: 2021-12-08
Description:

Abstract

Objectives: The objective of this study was to investigate the association between p53 expression and microbial acetaldehyde production in patients with oral squamous cell carcinoma (OSCC).

Study design: Oral mucosal biopsies from 22 patients with OSCC and 24 healthy controls (HCs) were collected. p53 expression was analyzed by immunohistochemistry. Microbial samples were collected from the mucosa and microbial acetaldehyde production from ethanol was measured by gas chromatography.

Results: The majority of all OSCC (77%) and HC samples (67%) produced mutagenic levels of acetaldehyde (>100 µM). A significant positive correlation between microbial acetaldehyde production and p53 expression levels in OSCC samples was seen in the intermediate and superficial layers of the epithelium of the infiltrative zone (P = .0005 and P = .0004, respectively) and in the superficial layer of the healthy appearing mucosa next to the tumor (P = .0391). There was no significant correlation between acetaldehyde levels and p53 expression in HC samples.

Conclusions: Our results show an association between microbial acetaldehyde production and immunostaining of p53 in OSCC samples.

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Series: Oral surgery, oral medicine, oral pathology and oral radiology
ISSN: 2212-4403
ISSN-E: 2212-4411
ISSN-L: 2212-4403
Volume: 131
Issue: 5
Pages: 527 - 533
DOI: 10.1016/j.oooo.2020.11.015
OADOI: https://oadoi.org/10.1016/j.oooo.2020.11.015
Type of Publication: A1 Journal article – refereed
Field of Science: 313 Dentistry
Subjects:
Copyright information: © 2020 Elsevier Inc. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http:/creativecommons.org/licenses/by-nc-nd/4.0/
  https://creativecommons.org/licenses/by-nc-nd/4.0/