University of Oulu

Wahab, A., Hyytiäinen, A., Wahbi, W., Tuomainen, K., Tervo, S., Conesa‐Zamora, P., Jauhiainen, L., Mäkinen, L. K., Paavonen, T., Toppila‐Salmi, S., Salem, A., Almangush, A., Salo, T., & Al‐Samadi, A. (2021). The effect of fascin 1 inhibition on head and neck squamous cell carcinoma cells. European Journal of Oral Sciences, 129(6).

The effect of fascin 1 inhibition on head and neck squamous cell carcinoma cells

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Author: Wahab, Awais1,2; Hyytiäinen, Aini1,2; Wahbi, Wafa1,2;
Organizations: 1Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, Helsinki, Finland
2Translational Immunology Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland
3Haartman Institute, University of Helsinki, Helsinki, Finland
4Department of Pathology, Faculty of Medicine and Health Technology and Fimlab Laboratories, Tampere University and Tampere University Hospital, Tampere, Finland
5Pathology and Histology Department, Health Faculty, Universidad Católica de Murcia, Campus de los Jerónimos, Guadalupe, Murcia, Spain
6Clinical Analysis Department, Group of Molecular Pathology and Pharmacogenetics, Biomedical Research Institute Murcia, Hospital Universitario Santa Lucía, Cartagena, Spain
7Department of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
8Skin and Allergy Hospital, Helsinki University Hospital and University of Helsinki, Helsinki, Finland
9Department of Pathology, University of Helsinki, Helsinki, Finland
10Research Program in Systems Oncology, Faculty of Medicine, University of Helsinki, Helsinki, Finland
11Institute of Biomedicine, Pathology, University of Turku, Turku, Finland
12Cancer Research and Translational Medicine Research Unit, University of Oulu, Oulu, Finland
13Medical Research Center Oulu, Oulu University HospitalUniversity of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.2 MB)
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Language: English
Published: John Wiley & Sons, 2021
Publish Date: 2022-08-04


Fascin 1 plays important pro-metastatic roles in head and neck carcinoma (HNSCC) migration, invasion, and metastasis. However, limited advancement in targeting metastasis remains a major obstacle in improving HNSCC patients’ survival. Therefore, we assessed the therapeutic potential of fascin 1 targeted inhibition and its potential prognostic value in HNSCC patients. Using in vitro and in vivo approaches, we investigated the effect of compound G2, a novel fascin 1 inhibitor, on HNSCC cells migration, invasion, and metastasis. High-throughput screening (HTS) was used to assess cytotoxic activity of compound G2 alone or combined with irradiation. We also evaluated the prognostic potential of fascin 1 in HNSCC patients. Interestingly, compound G2 reduced carcinoma cells migration and invasion in vitro and inhibited metastasis in vivo. Moreover, HTS revealed a modest cytotoxic activity of the compound G2 on HNSCC cell lines. Irradiation did not synergistically enhance the compound G2-mediated cytotoxic activity. Survival analyses showed that high fascin 1 immunoexpression, at the tumor invasive front, was associated with cancer-specific mortality in the advanced stages of HNSCC. Collectively, our findings suggest that fascin 1 represents a promising anti-metastatic therapeutic target and a useful prognostic marker in patients with HNSCC. Novel anti-metastatic agents could provide a valuable addition to cancer therapy.

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Series: European journal of oral sciences
ISSN: 0909-8836
ISSN-E: 1600-0722
ISSN-L: 0909-8836
Volume: 129
Issue: 6
Article number: e12819
DOI: 10.1111/eos.12819
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
Funding: This research was funded by Sigrid Jusélius Foundation, the Cancer Society of Finland, Instituto de Salud Carlos III (Spanish Ministry of Health) and FEDER funds (ref: PI18/00144), the Oulu University Hospital MRC grant, the Helsinki University Central Hospital research funds, Research Fund of Finnish Otolaryngological Society, and Jane and Aatos Erkko Foundation.
Copyright information: © 2021 The Authors. European Journal of Oral Sciences published by JohnWiley & Sons Ltd on behalf of Scandinavian Division of the International Association for Dental Research. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.