Maleckar, M. M., Martín-Vasallo, P., Giles, W. R., & Mobasheri, A. (2020). Physiological Effects of the Electrogenic Current Generated by the Na+/K+Pump in Mammalian Articular Chondrocytes. Bioelectricity, 2(3), 258–268. https://doi.org/10.1089/bioe.2020.0036
Physiological effects of the electrogenic current generated by the Na⁺/K⁺ pump in mammalian articular chondrocytes
|Author:||Maleckar, Mary M.1; Martín-Vasallo, Pablo2,3; Giles, Wayne R.4;|
1Simula Research Laboratory, Inc., Oslo, Norway
2UD of Biochemistry and Molecular Biology, Universidad de La Laguna, San Cristóbal de La Laguna, Spain
3Instituto de Tecnologías Biomédicas de Canarias, Universidad de La Laguna, San Cristóbal de La Laguna, Spain
4Department of Physiology and Pharmacology, University of Calgary, Calgary, Canada
5Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
6Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania
7Department of Orthopedics, Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
|Online Access:||PDF Full Text (PDF, 0.6 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe202201031023
Mary Ann Liebert,
|Publish Date:|| 2022-01-03
Background: Although the chondrocyte is a nonexcitable cell, there is strong interest in gaining detailed knowledge of its ion pumps, channels, exchangers, and transporters. In combination, these transport mechanisms set the resting potential, regulate cell volume, and strongly modulate responses of the chondrocyte to endocrine agents and physicochemical alterations in the surrounding extracellular microenvironment.
Materials and methods: Mathematical modeling was used to assess the functional roles of energy-requiring active transport, the Na⁺/K⁺ pump, in chondrocytes.
Results: Our findings illustrate plausible physiological roles for the Na⁺/K⁺ pump in regulating the resting membrane potential and suggest ways in which specific molecular components of pump can respond to the unique electrochemical environment of the chondrocyte.
Conclusion: This analysis provides a basis for linking chondrocyte electrophysiology to metabolism and yields insights into novel ways of manipulating or regulating responsiveness to external stimuli both under baseline conditions and in chronic diseases such as osteoarthritis.
|Pages:||258 - 268|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
318 Medical biotechnology
A.M. has received funding from the following sources: The European Commission Framework 7 programme (EU FP7; HEALTH.2012.2.4.5-2, project No. 305815; Novel Diagnostics and Biomarkers for Early Identification of Chronic Inflammatory Joint Diseases). The Innovative Medicines Initiative Joint Undertaking under grant agreement number 115770, resources of which are composed of financial contribution from the European Union's Seventh Framework programme (FP7/2007–2013) and European Federation of Pharmaceutical Industries and Associations companies' in-kind contribution. A.M. also wishes to acknowledge funding from the European Commission through a Marie Curie Intra-European Fellowship for Career Development grant (project No. 625746; acronym: CHONDRION; FP7-PEOPLE-2013-IEF). A.M. also wishes to acknowledge financial support from the European Structural and Social Funds (ES Struktūrinės Paramos) through the Research Council of Lithuania (Lietuvos Mokslo Taryba) according to the activity “Improvement of researchers” qualification by implementing world-class R&D projects' of Measure number 09.3.3-LMT-K-712 (grant application code: 09.3.3-LMT-K-712-01-0157, agreement No. DOTSUT-215) and the new funding programme: Attracting Foreign Researchers for Research Implementation (2018–2022). M.M. would like to acknowledge funding from Norway's Ministry of Education and Research via her position at Simula Research Laboratory.
© Mary M. Maleckar et al. 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any non-commercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.