University of Oulu

Piavchenko, G., Kozlov, I., Dremin, V., Stavtsev, D., Seryogina, E., Kandurova, K., Shupletsov, V., Lapin, K., Alekseyev, A., Kuznetsov, S., Bykov, A., Dunaev, A., Meglinski, I., J. Biophotonics 2021, 14( 12), e202100216. https://doi.org/10.1002/jbio.202100216

Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

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Author: Piavchenko, Gennadii1,2,3; Kozlov, Igor4; Dremin, Viktor3,4,5;
Organizations: 1Department of Histology, Cytology and Embryology, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
2V.A. Negovsky Scientific Research Institute of General Reanimatology, Federal Research and Clinical Centre of Intensive Care Medicine and Rehabilitology, Moscow, Russia
3Cell Physiology and Pathology Laboratory, Orel State University, Orel, Russia
4R&D Center of Biomedical Photonics, Orel State University, Orel, Russia
5College of Engineering and Physical Sciences, Aston University, Birmingham, UK
6Institute of Biomedical Systems, National Research University of Electronic Technology (MIET), Zelenograd, Russia
7Opto-Electronics and Measurement Techniques, Faculty of Information and Electrical Engineering, University of Oulu, Oulu, Finland
8Immanuel Kant Baltic Federal University, Kaliningrad, Russia
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe202201199454
Language: English
Published: John Wiley & Sons, 2021
Publish Date: 2022-01-19
Description:

Abstract

The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi-modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood flow in small and medium size vessels during a few minutes of respiratory arrest, while the same effect was observed in large sinuses and their branches during the circulatory cessation. Concurrently, the redox ratio assessed with fluorescence spectroscopy indicates progressing hypoxia, NADH accumulation and increase of FAD consumption. The results of diffuse reflectance spectra measurements display a more rapid grow of the perfusion of deoxygenated blood in case of circulatory impairment. In addition, consequent histopathological analysis performed by using new tissue staining procedure developed in-house. It shows notably higher reduction of size of the neurons due to their wrinkling within brain tissues influenced by circulation impair. Whereas, the brain tissues altered with the respiratory arrest demonstrate focal perivascular oedema and mild hypoxic changes of neuronal morphology. Thus, the study suggests that consequences of a cessation of cerebral blood flow become more dramatic and dangerous compare to respiratory arrest.

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Series: Journal of biophotonics
ISSN: 1864-063X
ISSN-E: 1864-0648
ISSN-L: 1864-063X
Volume: 14
Issue: 12
Article number: e202100216
DOI: 10.1002/jbio.202100216
OADOI: https://oadoi.org/10.1002/jbio.202100216
Type of Publication: A1 Journal article – refereed
Field of Science: 217 Medical engineering
114 Physical sciences
213 Electronic, automation and communications engineering, electronics
Subjects:
Funding: The authors disclose receipt of financial support for the research, authorship, and/or publication of this article as following: Gennadii Piavchenko and Sergey Kuznetsov acknowledge the support from the Ministry of Science and Higher Education of the Russian Federation within the framework of State support for the creation and development of World-Class research Centres “Digital Biodesign and Personalized Healthcare” No 075-15-2020-926. Viktor Dremin kindly acknowledges personal support from the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 839888. Andrey Dunaev and Igor Meglinski acknowledge funding from the Academy of Finland (grant No. 326204). This study has been also partially supported by the European Union's Horizon 2020 research and innovation programme under grant agreement No. 863214—NEUROPA project.
EU Grant Number: (863214) NEUROPA - Non-invasive dynamic neural control by laser-based technology
Academy of Finland Grant Number: 326204
Detailed Information: 326204 (Academy of Finland Funding decision)
Copyright information: © 2021 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
  https://creativecommons.org/licenses/by-nc-nd/4.0/