University of Oulu

Cheng, X, Vinokurov, AY, Zherebtsov, EA, et al. Variability of mitochondrial energy balance across brain regions. J Neurochem. 2021; 157: 1234– 1243. https://doi.org/10.1111/jnc.15239

Variability of mitochondrial energy balance across brain regions

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Author: Cheng, XinPing1,2; Vinokurov, Andrey Y.3; Zherebtsov, Evgeniy A.3,4;
Organizations: 1Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, London, UK
2CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei, China
3Cell Physiology and Pathology Laboratory, Orel State University, Orel, Russia
4Optoelectronics and Measurement Techniques Laboratory, University of Oulu, Oulu, Finland
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe202201199479
Language: English
Published: John Wiley & Sons, 2021
Publish Date: 2022-05-21
Description:

Abstract

Brain is not homogenous and neurons from various brain regions are known to have different vulnerabilities to mitochondrial mutations and mitochondrial toxins. However, it is not clear if this vulnerability is connected to different energy metabolism in specific brain regions. Here, using live-cell imaging, we compared mitochondrial membrane potential and nicotinamide adenine dinucleotide (NADH) redox balance in acute rat brain slices in different brain regions and further detailed the mitochondrial metabolism in primary neurons and astrocytes from rat cortex, midbrain and cerebellum. We have found that mitochondrial membrane potential is higher in brain slices from the hippocampus and brain stem. In primary co-cultures, mitochondrial membrane potential in astrocytes was lower than in neurons, whereas in midbrain cells it was higher than in cortex and cerebellum. The rate of NADH production and mitochondrial NADH pool were highest in acute slices from midbrain and midbrain primary neurons and astrocytes. Although the level of adenosine tri phosphate (ATP) was similar among primary neurons and astrocytes from cortex, midbrain and cerebellum, the rate of ATP consumption was highest in midbrain cells that lead to faster neuronal and astrocytic collapse in response to inhibitors of ATP production. Thus, midbrain neurons and astrocytes have a higher metabolic rate and ATP consumption that makes them more vulnerable to energy deprivation.

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Series: Journal of neurochemistry
ISSN: 0022-3042
ISSN-E: 1471-4159
ISSN-L: 0022-3042
Volume: 157
Issue: 4
Pages: 1234 - 1243
DOI: 10.1111/jnc.15239
OADOI: https://oadoi.org/10.1111/jnc.15239
Type of Publication: A1 Journal article – refereed
Field of Science: 3112 Neurosciences
Subjects:
ATP
Funding: This work was supported by the grant of the Russian Federation Government Nr. 075-15-2019-1877. E.Z. acknowledges the support of the Academy of Finland (Grant No. 318281).
Academy of Finland Grant Number: 318281
Detailed Information: 318281 (Academy of Finland Funding decision)
Copyright information: © 2020 International Society for Neurochemistry. This is the peer reviewed version of the following article: Cheng, X, Vinokurov, AY, Zherebtsov, EA, et al. Variability of mitochondrial energy balance across brain regions. J Neurochem. 2021; 157: 1234– 1243, which has been published in final form at https://doi.org/10.1111/jnc.15239. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.