University of Oulu

Shvetsova, A., Masud, A. J., Schneider, L., Bergmann, U., Monteuuis, G., Miinalainen, I. J., Hiltunen, J. K., & Kastaniotis, A. J. (2021). A hunt for OM45 synthetic petite interactions in Saccharomyces cerevisiae reveals a role for Miro GTPase Gem1p in cristae structure maintenance. MicrobiologyOpen, 10, e1238. https://doi.org/10.1002/mbo3.1238

A hunt for OM45 synthetic petite interactions in Saccharomyces cerevisiae reveals a role for Miro GTPase Gem1p in cristae structure maintenance

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Author: Shvetsova, Antonina1; Masud, Ali J.1; Schneider, Laura1;
Organizations: 1Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.5 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2022013111302
Language: English
Published: John Wiley & Sons, 2021
Publish Date: 2022-01-31
Description:

Abstract

Om45 is a major protein of the yeast’s outer mitochondrial membrane under respiratory conditions. However, the cellular role of the protein has remained obscure. Previously, deletion mutant phenotypes have not been found, and clear amino acid sequence similarities that would allow inferring its functional role are not available. In this work, we describe synthetic petite mutants of GEM1 and UGO1 that depend on the presence of OM45 for respiratory growth, as well as the identification of several multicopy suppressors of the synthetic petite phenotypes. In the analysis of our mutants, we demonstrate that Om45p and Gem1p have a collaborative role in the maintenance of mitochondrial morphology, cristae structure, and mitochondrial DNA maintenance. A group of multicopy suppressors rescuing the synthetic lethal phenotypes of the mutants on non-fermentable carbon sources additionally supports this result. Our results imply that the synthetic petite phenotypes we observed are due to the disturbance of the inner mitochondrial membrane and point to this mitochondrial sub-compartment as the main target of action of Om45p, Ugo1p, and the yeast Miro GTPase Gem1p.

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Series: MicrobiologyOpen
ISSN: 2045-8827
ISSN-E: 2045-8827
ISSN-L: 2045-8827
Volume: 10
Issue: 5
Article number: e1238
DOI: 10.1002/mbo3.1238
OADOI: https://oadoi.org/10.1002/mbo3.1238
Type of Publication: A1 Journal article – refereed
Field of Science: 1183 Plant biology, microbiology, virology
1182 Biochemistry, cell and molecular biology
Subjects:
Funding: This work was carried out at the Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu of the University of Oulu, Finland. Financial support was provided by Biocenter Oulu, the Academy of Finland (#138690 and #285945), and the Sigrid Jusélius Foundation.
Academy of Finland Grant Number: 138690
Detailed Information: 138690 (Academy of Finland Funding decision)
Copyright information: © 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
  https://creativecommons.org/licenses/by-nc-nd/4.0/