University of Oulu

Podolich, O., Prekrasna, I., Parnikoza, I., Voznyuk, T., Zubova, G., Zaets, I., Miryuta, N., Myryuta, G., Poronnik, O., Kozeretska, I., Kunakh, V., Pirttila, A. M., Dykyi, E., & Kozyrovska, N. (2021). First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. From two distant localities of the maritime Antarctic. Czech Polar Reports, 11(1), 134–153. https://doi.org/10.5817/CPR2021-1-10

First record of the endophytic bacteria of Deschampsia antarctica Ė. Desv. from two distant localities of the maritime Antarctic

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Author: Podolich, Olga1; Prekrasna, Ievgeniia2; Parnikoza, Ivan1,2,3;
Organizations: 1Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine, Zabolotnogo Str. 150, Kyiv, 03680, Ukraine
2State Institution National Antarctic Scientific Center, Ministry of Education and Science of Ukraine, Shevchenko Ave 16, Kyiv, 01601, Ukraine
3National University of Kyiv-Mohyla Academy, Hryhoriya Skovorody St 2, Kyiv, 04655, Ukraine
4Department of Ecology and Genetics, University of Oulu, FIN - 90014 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.1 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2022020817976
Language: English
Published: Masaryk University Press, 2021
Publish Date: 2022-02-08
Description:

Abstract

Endophytic bacteria, recognized for their beneficial effects on plant development and adaptation, can facilitate the survival of Antarctic plants in severe environments. Here we studied endophytes of the vascular plant Deschampsia antarctica Ė. Desv. from two distantly located regions in the maritime Antarctic: King George Island (South Shetland Islands) and Galindez Island (Argentine Islands). Bacterial group-specific PCR indicated presence of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, Cytophaga-Flavobacteria and Actinobacteria in root and leaf endosphere of D. antarctica sampled at four distinct sites of both locations. The diversity of endophytic bacteria was significantly higher in the leaves compared to the roots in plants from Galindez Island. Similarly, the diversity of endophytes was higher in the leaves rather than roots of plants from the King George Island. Twelve bacterial species were isolated from roots of D. antarctica of Galindez Island (the Karpaty Ridge and the Meteo Point) and identified by sequencing the 16S rRNA gene. Isolates were dominated by the Pseudomonas genus, followed by the genera Bacillus and Micrococcus. The vast majority of the isolates exhibited cellulase and pectinase activities, however, Bacillus spp. expressed neither of them, suggesting lack of genetic flow of these traits in endophytic bacilli in the maritime Antarctic. Pseudomonas sp. IMBG305 promoted an increase in the leaf number in most of the treated plant genotypes when compared with non-inoculated plants, and a rapid vegetation period of D. antarctica cultured in vitro, albeit the length of leaves in the treated plants was significantly lower, and flavonoid content leveled off in all treated plants. D. antarctica is known to develop diverse ecotypes with regard to ecological conditions, such as organic input, moisture or wind exposition. The D. antarctica phenotype could be extended further through the endophyte colonization, since phenotypic changes were observed in the inoculated D. antarctica plants grown in vitro in our study. Herewith, endophytes can contribute to plant phenotypic plasticity, potentially beneficial for adaptation of D. antarctica.

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Series: Czech polar reports
ISSN: 1805-0689
ISSN-E: 1805-0697
ISSN-L: 1805-0689
Volume: 11
Issue: 1
Pages: 134 - 153
DOI: 10.5817/CPR2021-1-10
OADOI: https://oadoi.org/10.5817/CPR2021-1-10
Type of Publication: A1 Journal article – refereed
Field of Science: 1183 Plant biology, microbiology, virology
Subjects:
Funding: The fieldwork was supported by the National Antarctic Scientific Centre of the Ministry of Science of Ukraine during the 18th Ukrainian Antarctic expeditions and approved by Department of Antarctic study of Institute of Biochemistry and Biophysics of Polish Academy of Sciences (PAS). We would like to thank Dr. Papitashvili for his help in the expedition preparation, Ms. A. Berezkina for her valuable help in the map preparation and Mr. Mag. M. Wierzgoń for the sample collection. This study was carried out as a part of the State Priority Scientific and Technical Research Program on the Antarctic during 2011-2020 within the NASU and PAS joint 2015-2017 project “Adaptive strategies of mutual survival of organisms in extreme environments”.
Copyright information: © 2020 Czech Polar Reports. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
  https://creativecommons.org/licenses/by-nc-nd/4.0/