University of Oulu

Aatir A. Tungekar, Angel Castillo-Corujo, Lloyd W. Ruddock; So you want to express your protein in Escherichia coli?. Essays Biochem 26 July 2021; 65 (2): 247–260. doi:

So you want to express your protein in Escherichia coli?

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Author: Tungekar, Aatir A.1; Castillo-Corujo, Angel1; Ruddock, Lloyd W.1
Organizations: 1Protein and Structural Biology Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu 90220, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.6 MB)
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Language: English
Published: Portland Press, 2021
Publish Date: 2022-02-24


Recombinant proteins have been extensively employed as therapeutics for the treatment of various critical and life-threatening diseases and as industrial enzymes in high-value industrial processes. Advances in genetic engineering and synthetic biology have broadened the horizon of heterologous protein production using multiple expression platforms. Selection of a suitable expression system depends on a variety of factors ranging from the physicochemical properties of the target protein to economic considerations. For more than 40 years, Escherichia coli has been an established organism of choice for protein production. This review aims to provide a stepwise approach for any researcher embarking on the journey of recombinant protein production in E. coli. We present an overview of the challenges associated with heterologous protein expression, fundamental considerations connected to the protein of interest (POI) and designing expression constructs, as well as insights into recently developed technologies that have contributed to this ever-growing field.

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Series: Essays in biochemistry
ISSN: 0071-1365
ISSN-E: 1744-1358
ISSN-L: 0071-1365
Volume: 65
Issue: 2
Pages: 247 - 260
DOI: 10.1042/EBC20200170
Type of Publication: A2 Review article in a scientific journal
Field of Science: 1182 Biochemistry, cell and molecular biology
Funding: This work was supported the European Union’s Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie [grant number 642937].
EU Grant Number: (642937) RENALTRACT - Development and disease of the renal tract
Copyright information: © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.