University of Oulu

Kashif M, Jurvansuu J, Hyder R, Vainio EJ and Hantula J (2021) Phenotypic Recovery of a Heterobasidion Isolate Infected by a Debilitation-Associated Virus Is Related to Altered Host Gene Expression and Reduced Virus Titer. Front. Microbiol. 12:661554. doi:10.3389/fmicb.2021.661554

Phenotypic recovery of a Heterobasidion isolate infected by a debilitation-associated virus is related to altered host gene expression and reduced virus titer

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Author: Kashif, Muhammad1; Jurvansuu, Jaana2; Hyder, Rafiqul1;
Organizations: 1Natural Resources Institute Finland, Helsinki, Finland
2Department of Biology, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1 MB)
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Language: English
Published: Frontiers Media, 2021
Publish Date: 2022-02-28


The fungal genus Heterobasidion includes forest pathogenic species hosting a diverse group of partitiviruses. They include the host debilitating Heterobasidion partitivirus 13 strain an1 (HetPV13‐an1), which was originally observed in a slowly growing H. annosum strain 94233. In this study, a relatively fast-growing sector strain 94233‐RC3 was isolated from a highly debilitated mycelial culture of 94233, and its gene expression and virus transcript quantities as well as the genomic sequence of HetPV13‐an1 were examined. The sequence of HetPV13‐an1 genome in 94233‐RC3 was identical to that in the original 94233, and thus not the reason for the partial phenotypic recovery. According to RNA-seq analysis, the HetPV13‐an1 infected 94233‐RC3 transcribed eight genes differently from the partitivirus-free 94233‐32D. Three of these genes were downregulated and five upregulated. The number of differentially expressed genes was considerably lower and the changes in their expression were small compared to those of the highly debilitated original strain 94233 with the exception of the most highly upregulated ones, and therefore viral effects on the host transcriptome correlated with the degree of the virus‐caused debilitation. The amounts of RdRp and CP transcripts of HetPV13‐an1 were considerably lower in 94233‐RC3 and also in 94233 strain infected by a closely related mildly debilitating virus HetPV13‐an2, suggesting that the virus titer would have a role in determining the effect of HetPV13 viruses on their hosts.

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Series: Frontiers in microbiology
ISSN: 1664-302X
ISSN-E: 1664-302X
ISSN-L: 1664-302X
Volume: 12
Article number: 661554
DOI: 10.3389/fmicb.2021.661554
Type of Publication: A1 Journal article – refereed
Field of Science: 1183 Plant biology, microbiology, virology
1181 Ecology, evolutionary biology
Funding: This work was supported by the Academy of Finland (grant numbers 322001 and 309896).
Copyright information: © 2021 Kashif, Jurvansuu, Hyder, Vainio and Hantula. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.