University of Oulu

Kopra E, Lahdentausta L, Pietiäinen M, Buhlin K, Mäntylä P, Hörkkö S, Persson R, Paju S, Sinisalo J, Salminen A and Pussinen PJ (2021) Systemic Antibiotics Influence Periodontal Parameters and Oral Microbiota, But Not Serological Markers. Front. Cell. Infect. Microbiol. 11:774665. doi: 10.3389/fcimb.2021.774665

Systemic antibiotics influence periodontal parameters and oral microbiota, but not serological markers

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Author: Kopra, Elisa1; Lahdentausta, Laura1; Pietiäinen, Milla1;
Organizations: 1Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
2Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden
3Institute of Dentistry, University of Eastern Finland, Kuopio, Finland
4Oral and Maxillofacial Diseases, Kuopio University Hospital, Kuopio, Finland
5Medical Microbiology and Immunology, Research Unit of Biomedicine, University of Oulu, Oulu, Finland
6Medical Research Center, Oulu University Hospital and University of Oulu, Oulu, Finland
7Department of Periodontics, University of Washington, Seattle, WA, United States
8Department of Oral Medicine, University of Washington, Seattle, WA, United States
9Faculty of Health Sciences, Kristianstad University, Kristianstad, Sweden
10Division of Cardiology, Heart and Lung Center, Department of Medicine, Helsinki University Hospital, Helsinki, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.3 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2022030121365
Language: English
Published: Frontiers Media, 2021
Publish Date: 2022-03-01
Description:

Abstract

The use of systemic antibiotics may influence the oral microbiota composition. Our aim was to investigate in this retrospective study whether the use of prescribed antibiotics associate with periodontal status, oral microbiota, and antibodies against the periodontal pathogens. The Social Insurance Institution of Finland Data provided the data on the use of systemic antibiotics by record linkage to purchased medications and entitled reimbursements up to 1 year before the oral examination and sampling. Six different classes of antibiotics were considered. The Parogene cohort included 505 subjects undergoing coronary angiography with the mean (SD) age of 63.4 (9.2) years and 65% of males. Subgingival plaque samples were analysed using the checkerboard DNA-DNA hybridisation. Serum and saliva antibody levels to periodontal pathogens were analysed with immunoassays and lipopolysaccharide (LPS) activity with the LAL assay. Systemic antibiotics were prescribed for 261 (51.7%) patients during the preceding year. The mean number of prescriptions among them was 2.13 (range 1–12), and 29.4% of the prescriptions were cephalosporins, 25.7% penicillins, 14.3% quinolones, 12.7% macrolides or lincomycin, 12.0% tetracycline, and 5.8% trimethoprim or sulphonamides. In linear regression models adjusted for age, sex, current smoking, and diabetes, number of antibiotic courses associated significantly with low periodontal inflammation burden index (PIBI, p < 0.001), bleeding on probing (BOP, p = 0.006), and alveolar bone loss (ABL, p = 0.042). Cephalosporins associated with all the parameters. The phyla mainly affected by the antibiotics were Bacteroidetes and Spirochaetes. Their levels were inversely associated with the number of prescriptions (p = 0.010 and p < 0.001) and directly associated with the time since the last prescription (p = 0.019 and p < 0.001). Significant inverse associations were observed between the number of prescriptions and saliva concentrations of Prevotella intermedia, Tannerella forsythia, and Treponema denticola and subgingival bacterial amounts of Porphyromonas gingivalis, P. intermedia, T. forsythia, and T. denticola. Saliva or serum antibody levels did not present an association with the use of antibiotics. Both serum (p = 0.031) and saliva (p = 0.032) LPS activity was lower in patients having any antibiotic course less than 1 month before sampling. Systemic antibiotics have effects on periodontal inflammation and oral microbiota composition, whereas the effects on host immune responses against the periodontal biomarker species seem unchanged.

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Series: Frontiers in cellular and infection microbiology
ISSN: 2235-2988
ISSN-E: 2235-2988
Volume: 11
Article number: 774665
DOI: 10.3389/fcimb.2021.774665
OADOI: https://oadoi.org/10.3389/fcimb.2021.774665
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
313 Dentistry
Subjects:
Funding: P.J.P. received funding from the Finnish Dental Society Apollonia, the Yrjö Jahnsson foundation, the Sigrid Juselius foundation, the Aarne Koskelo foundation, the Foundation for Cardiovascular research, and the Academy of Finland (#1340750).
Copyright information: © 2021 Kopra, Lahdentausta, Pietiäinen, Buhlin, Mäntylä, Hörkkö, Persson, Paju, Sinisalo, Salminen and Pussinen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
  https://creativecommons.org/licenses/by/4.0/