University of Oulu

Korpela, K., Renko, M., Paalanne, N. et al. Microbiome of the first stool after birth and infantile colic. Pediatr Res 88, 776–783 (2020).

Microbiome of the first stool after birth and infantile colic

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Author: Kielenniva, Katja1; Renko, Marjo2,3; Paalanne, Niko1,2;
Organizations: 1PEDEGO Research Unit and Medical Research Centre Oulu, University of Oulu, Oulu, Finland
2Department of Pediatrics and Adolescence, Oulu University Hospital, Oulu, Finland
3Department of Pediatrics, University of Eastern Finland, Kuopio, Finland
4Ecology and Genetics, Faculty of Science, University of Oulu, Oulu, Finland
5Genobiomics Ltd, Oulu, Finland
6Biocenter Oulu, University of Oulu, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.1 MB)
Persistent link:
Language: English
Published: Springer Nature, 2020
Publish Date: 2022-03-01


Background: Recent studies have shown a diverse microbiome in the first stool after birth. The clinical significance of the microbiome of the first stool is not known. Infantile colic has earlier been associated with the composition of the intestinal microbiome.

Methods: We set out to test whether the microbiome of the first stool is associated with subsequent infantile colic in a prospective, population-based cohort study of 212 consecutive newborn infants. We used next-generation sequencing of the bacterial 16S rRNA gene.

Results: The newborns who later developed infantile colic (n = 19) had a lower relative abundance of the genus Lactobacillus and the phylum Firmicutes in the first stool than those who remained healthy (n = 139). By using all microbiome data, random forest algorithm classified newborn with subsequent colic and those who remained healthy with area under the curve of 0.66 (SD 0.03) as compared to that of shuffled samples (P value <0.001).

Conclusions: In this prospective, population-based study, the microbiome of the first-pass meconium was associated with subsequent infantile colic. Our results suggest that the pathogenesis of infantile colic is closely related to the intestinal microbiome at birth.

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Series: Pediatric research
ISSN: 0031-3998
ISSN-E: 1530-0447
ISSN-L: 0031-3998
Volume: 88
Pages: 776 - 783
DOI: 10.1038/s41390-020-0804-y
Type of Publication: A1 Journal article – refereed
Field of Science: 3123 Gynaecology and paediatrics
Funding: The following institutions financially supported the study: Pediatric Research Foundation, Finland; Academy of Finland; Juho Vainio Foundation, Finland; Alma och K.A. Snellman Stiftelsen, Finland; UniOGS Graduate School, University of Oulu, Finland; and Emil Aaltonen Foundation, Finland.
Copyright information: © International Pediatric Research Foundation, Inc. 2020. This is a post-peer-review, pre-copyedit version of an article published in Pediatr Res. The final authenticated version is available online at