University of Oulu

Myllymäki SM., Liu X., Varjosalo M., Manninen A. (2021) Proximity-Dependent Biotinylation (BioID) of Integrin Interaction Partners. In: Vicente-Manzanares M. (eds) The Integrin Interactome. Methods in Molecular Biology, vol 2217. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0962-0_6

Proximity-dependent biotinylation (BioID) of integrin interaction partners

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Author: Myllymäki, Satu-Marja1,2; Liu, Xiaonan2; Varjosalo, Markku2;
Organizations: 1Oulu Center for Cell-Matrix Research, Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
2HiLIFE – Institute of Biotechnology, University of Helsinki, Helsinki, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 4 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2022030321736
Language: English
Published: Humana Press, 2021
Publish Date: 2022-03-03
Description:

Abstract

Integrins are heterodimeric adhesion receptors that maintain cell–extracellular matrix (ECM) interactions in diverse tissue microenvironments. They mediate cell adhesion and signaling through the assembly of large cytoplasmic multiprotein complexes that focally connect with the cytoskeleton. Integrin adhesion complexes (IAC) are specialized by the type of integrin-ECM contact and are sensitive to mechanical forces. Thus, they encrypt context-dependent information about the microenvironment in their composition. Signals mediated through IACs modulate many aspects of cell behavior, which allows cells to adapt to their surroundings. To gain insights into their function, IACs have been isolated from cultured cells and explored by proteomics. IACs are insoluble by nature and held together by transient/weak interactions, which makes it challenging to isolate intact IACs. Usually all IACs coupled to a specified ECM, which may employ different integrins, are isolated. Here we describe an alternative method based on proximity-dependent biotin identification (BioID), where specific integrin interaction partners are labeled in live cells and isolated without the need to isolate intact IACs.

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Series: Methods in molecular biology
ISSN: 1064-3745
ISSN-E: 1940-6029
ISSN-L: 1064-3745
ISBN: 978-1-0716-0962-0
ISBN Print: 978-1-0716-0961-3
Volume: 2217
Pages: 57 - 69
DOI: 10.1007/978-1-0716-0962-0_6
OADOI: https://oadoi.org/10.1007/978-1-0716-0962-0_6
Host publication: The integrin interactome : methods and protocols
Host publication editor: Vicente-Manzanares, Miguel
Type of Publication: A3 Book chapter
Field of Science: 1182 Biochemistry, cell and molecular biology
Subjects:
Copyright information: © Springer Science+Business Media, LLC, part of Springer Nature 2021. This is a post-peer-review, pre-copyedit version of an article published in The Integrin Interactome. Methods in Molecular Biology. The final authenticated version is available online at https://doi.org/10.1007/978-1-0716-0962-0_6.