University of Oulu

Saukko, AEA, Nykänen, O, Sarin, JK, et al. Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro. J Orthop Res. 2022; 40: 703- 711. https://doi.org/10.1002/jor.25066

Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro

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Author: Saukko, Annina E. A.1,2; Nykänen, Olli1,3; Sarin, Jaakko K.1,4;
Organizations: 1Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
2Department of Medical Physics, Turku University Hospital, Turku, Finland
3Research Unit of Medical Imaging Physics and Technology, University of Oulu, Oulu, Finland
4Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland
5Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
6Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Delft, The Netherlands
7Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands
8Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, Boston, Massachusetts, USA
9School of Information Technology and Electrical Engineering, The University of Queensland, Brisbane, Australia
10Science Service Center, Kuopio University Hospital, Kuopio, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.2 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2022030922692
Language: English
Published: John Wiley & Sons, 2022
Publish Date: 2022-03-09
Description:

Abstract

To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning. Posttraumatic changes include proteoglycan (PG) loss and elevated water content. Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes. Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture. Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies. Control samples were collected from three nonoperated ponies. The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points. As a reference, equilibrium moduli, dynamic moduli, and PG content were measured. Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min. Significant Spearman’s rank correlations were obtained at 20 and 24 h (ρ = 0.482–0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content. QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.

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Series: Journal of orthopaedic research
ISSN: 0736-0266
ISSN-E: 1554-527X
ISSN-L: 0736-0266
Volume: 40
Issue: 3
Pages: 703 - 711
DOI: 10.1002/jor.25066
OADOI: https://oadoi.org/10.1002/jor.25066
Type of Publication: A1 Journal article – refereed
Field of Science: 3126 Surgery, anesthesiology, intensive care, radiology
114 Physical sciences
Subjects:
Funding: Amit N. Patwa, PhD is acknowledged for preparing the CA4+. The work was supported by Jorma ja Märtha Sihvola Foundation, Academy of Finland (projects 307932, 285909 and 293970), Doctoral Program in Science, Technology and Computing (SCITECO, University of Eastern Finland), the Jenny and Antti Wihuri Foundation, and the Dutch Arthritis Association (Projects LLP-12 and LLP-22). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Copyright information: © 2021 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  https://creativecommons.org/licenses/by/4.0/